ID | 62856 |
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Author |
Imran, Shahin
Institute of Plant Science and Resources, Okayama University
Tsuchiya, Yoshiyuki
Institute of Plant Science and Resources, Okayama University
Tran, Sen Thi Huong
Institute of Plant Science and Resources, Okayama University
Katsuhara, Maki
Institute of Plant Science and Resources, Okayama University
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Abstract | In rice, the high-affinity K+ transporter, OsHKT1;3, functions as a Na+-selective transporter. mRNA variants of OsHKT1;3 have been reported previously, but their functions remain unknown. In this study, five OsHKT1;3 variants (V1-V5) were identified from japonica rice (Nipponbare) in addition to OsHKT1;3_FL. Absolute quantification qPCR analyses revealed that the transcript level of OsHKT1;3_FL was significantly higher than other variants in both the roots and shoots. Expression levels of OsHKT1;3_FL, and some variants, increased after 24 h of salt stress. Two electrode voltage clamp experiments in a heterologous expression system using Xenopus laevis oocytes revealed that oocytes expressing OsHKT1;3_FL and all of its variants exhibited smaller Na+ currents. The presented data, together with previous data, provide insights to understanding how OsHKT family members are involved in the mechanisms of ion homeostasis and salt tolerance in rice.
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Keywords | Na+ transport
rice
OsHKT1
3
mRNA variants
TEVC
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Published Date | 2021-09-24
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Publication Title |
Plants-Basel
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Volume | volume10
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Issue | issue10
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Publisher | MDPI
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Start Page | 2006
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ISSN | 2223-7747
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2021 by the authors.
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File Version | publisher
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PubMed ID | |
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Related Url | isVersionOf https://doi.org/10.3390/plants10102006
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License | https://creativecommons.org/licenses/by/4.0/
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Citation | Imran, S.; Tsuchiya, Y.; Tran, S.T.H.; Katsuhara, M. Identification and Characterization of Rice OsHKT1;3 Variants. Plants 2021, 10, 2006. https://doi.org/10.3390/ plants10102006
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Funder Name |
Ohara Foundation for Agricultural Science
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