ID | 58130 |
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Author |
Imran, Shahin
Institute of Plant Science and Resources, Okayama University
Horie, Tomoaki
Division of Applied Biology, Faculty of the Textile Science and Technology, Shinshu University
Katsuhara, Maki
Institute of Plant Science and Resources, Okayama University
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Abstract | OsHKT1;1 in rice, belongs to the high-affinity K+ Transporter family, has been found to be involved in salt tolerance. OsHKT1;1 in japonica rice (Nipponbare) produces mRNA variants, but their functions remain elusive. In salt tolerant rice, Pokkali, eight OsHKT1;1 variants (V1-V8) were identified in addition to the full-length OsHKT1;1 (FL) cDNA. Absolute quantification by qPCR revealed that accumulation of OsHKT1;1-FL mRNA is minor in contrast to that of OsHKT1;1-V1, -V2, -V4, and -V7 mRNAs, all of which are predominant in shoots, while only V1 and V7 mRNAs are predominant in roots. Two electrode voltage clamp (TEVC) experiments using Xenopus laevis oocytes revealed that oocytes-expressing OsHKT1;1-FL from Pokkali exhibited inward-rectified currents in the presence of 96 mM Na+ as reported previously. Further TEVC analyses indicated that six of eight OsHKT1;1 variants elicited currents in a Na+ or a K+ bath solution. OsHKT1;1-V6 exhibited a similar inward rectification to the FL protein. Contrastingly, however, the rests mediated bidirectional currents in both Na+ and K+ bath solutions. These data suggest possibilities that novel mechanisms regulating the transport activity of OsHKT1;1 might exist, and that OsHKT1;1 variants might also carry out distinct physiological roles either independently or in combination with OsHKT1;1-FL.
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Keywords | HKT
rice
salt tolerance
Na+ transport
K+ transport
mRNA variants
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Published Date | 2019-12-21
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Publication Title |
Plants
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Volume | volume9
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Issue | issue1
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Publisher | MDPI
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Start Page | 16
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ISSN | 2223-7747
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © 2019 by the authors.
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File Version | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
Related Url | isVersionOf https://doi.org/10.3390/plants9010016
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License | http://creativecommons.org/licenses/by/4.0/
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Funder Name |
Ministry of Education, Culture, Sports, Science and Technology
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