ID | 61704 |
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Mwaniki, Mercy W.
Graduate School of Environmental and Life Science, Okayama University
Mitalo, Oscar W.
Graduate School of Environmental and Life Science, Okayama University
Mworia, Eric G.
Graduate School of Environmental and Life Science, Okayama University
Owino, Willis O.
Graduate School of Environmental and Life Science, Okayama University
Hiwasa-Tanase, Kyoko
Graduate School of Life and Environmental Sciences, University of Tsukuba
Rose, Jocelyn K.C.
Plant Biology Section, School of Integrative Plant Science, Cornell University
Aoki, Koh
Graduate School of Life and Environmental Sciences, Osaka Prefecture University
Esumi, Tomoya
Academic Assembly Institute of Agricultural and Life Sciences, Shimane University
Kawai, Takashi
Graduate School of Environmental and Life Science, Okayama University
Nakano, Ryohei
Graduate School of Environmental and Life Science, Okayama University
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Ushijima, Koichiro
Graduate School of Environmental and Life Science, Okayama University
ORCID
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Kubo, Yasutaka
Graduate School of Environmental and Life Science, Okayama University
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Abstract | During ripening, European pear (Pyrus communis L. cv. ‘La France’) fruit undergo dramatic softening in response to increased ethylene production, whereas Chinese pear (Pyrus bretschneideri Rehd. cv. ‘Yali’) fruit remain firm, despite producing large amounts of ethylene. The molecular basis of this differential softening behavior is not well understood. In this study, we combined a yeast-based signal sequence trap (YSST) and macroarray gene expression analysis to identify putative genes encoding secreted proteins that control pear fruit softening. We identified 22 cDNAs annotated as encoding proteins with diverse cell wall-associated functions that were up- or down-regulated during fruit ripening in ‘La France’. Gene expression analysis in fruit that were treated with the ethylene perception inhibitor 1-methylcyclopropene (1-MCP) at 4 d after the onset of ripening revealed that 16 of the targeted genes are ethylene-regulated, while the others appear to be ethylene independent. Comparative gene expression analyses of ‘La France’ and ‘Yali’ fruit during ripening suggested that four ethylene-regulated cDNAs encoding cell wall modifying proteins, contig 2 (polygalacturonase 3), contig 15 (expansin), contig 19 (expansin) and contig 55 (pectate lyase) contribute to the different softening behaviors of ‘La France’ and ‘Yali’ fruit. Additionally, one ethylene-independent cell wall related gene, contig 36 (expansin), and three genes encoding proteins of unknown function, contigs 1, 13 and contig 75 showed differential expression between ‘La France’ and ‘Yali’ fruit during ripening. The results presented herein represent promising candidates for future functional analysis and elucidation of softening mechanisms.
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Keywords | YSST
‘La France’
‘Yali’
Polygalacturonase
Expansin
Pectate lyase
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Note | © 2020 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 License. http://creativecommons.org/licenses/by-nc-nd/4.0/.
This is the accepted manuscript version. The formal published version is available at [https://doi.org/10.1016/j.postharvbio.2020.111436] .
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Published Date | 2020-12-29
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Publication Title |
Postharvest Biology and Technology
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Volume | volume174
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Publisher | Elsevier
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Start Page | 111436
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ISSN | 09255214
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NCID | AA10838952
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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File Version | author
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DOI | |
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Related Url | isVersionOf https://doi.org/10.1016/j.postharvbio.2020.111436
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Citation | Mercy W. Mwaniki, Oscar W. Mitalo, Eric G. Mworia, Willis O. Owino, Kyoko Hiwasa-Tanase, Jocelyn K.C. Rose, Koh Aoki, Tomoya Esumi, Takashi Kawai, Ryohei Nakano, Koichiro Ushijima, Yasutaka Kubo, Combined signal sequence trap and macroarray analysis identifies genes associated with differential fruit softening characteristics during ripening in European and Chinese pears, Postharvest Biology and Technology, Volume 174, 2021, 111436, ISSN 0925-5214, https://doi.org/10.1016/j.postharvbio.2020.111436.
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Funder Name |
Japan Society for the Promotion of Science
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助成番号 | 16H04873
20H02977
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Open Access (Publisher) |
non-OA
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Open Archive (publisher) |
Non-OpenArchive
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