ID | 53002 |
FullText URL | |
Author |
Moritou, Yuki
Takeuchi, Yasuto
Seki, Hiroyuki
Nanba, Shintaro
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Abstract | We encountered a patient positive for anti-hepatitis C virus (HCV) whose serum HCV RNA was undetectable with the Roche AmpliPrep/Cobas TaqMan HCV assay (CAP/CTM) version 1 but showed a high viral load with the Abbott RealTime HCV assay (ART). Discrepancies in the detectability of serum HCV RNA were investigated among 891 consecutive patients who were positive for anti-HCV. Specific nucleotide variations causing the undetectability of HCV RNA were determined and confirmed by synthesizing RNA coding those variations. Serum samples with the discrepancies were also reassessed by CAP/CTM version 2. Among the 891 anti-HCV-positive patients, 4 patients had serum HCV RNA levels that were undetectable by CAP/CTM version 1 despite having levels of > 5 log IU/ml that were detected by ART. All four patients had HCV genotype 2a and high titers of anti-HCV. Sequencing of the HCV 5' noncoding regions revealed 2 common variations, A at nucleotide (nt) 145 and T at nt 151. Synthesized RNAs of the HCV 5' noncoding region with standard (NCR145G151C) and variant nucleotides at nt 145 and nt 151 were quantified with CAP/CTM. RNAs of NCR145G151C and NCR145G151T were quantifiable with CAP/CTM version 1, while those of NCR145A151T and NCR145A151C went undetected. The substitution from G to A at nt 145 specifically conferred this undetectability, while this undetectability was reverted in synthesized HCV RNA with correction of this variation. Reassessment of these samples by CAP/CTM version 2 resulted in similar levels of HCV RNA being detected by ART. We conclude that HCV patients with undetectable HCV RNA by CAP/CTM version 1 should be reassessed for viral quantification.
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Published Date | 2014-02
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Publication Title |
Journal of Clinical Microbiology
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Volume | volume52
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Issue | issue2
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Publisher | American Society for Microbiology
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Start Page | 544
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End Page | 548
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ISSN | 0095-1137
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NCID | AA00695531
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Content Type |
Journal Article
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Related Url | http://ousar.lib.okayama-u.ac.jp/metadata/52957
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language |
English
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Copyright Holders | Copyright © 2014, American Society for Microbiology. All Rights Reserved.
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File Version | author
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Refereed |
True
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DOI | |
Web of Science KeyUT |