ID | 57772 |
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Yamashita, Toru
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Shang, Jingwei
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Nakano, Yumiko
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Morihara, Ryuta
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Sato, Kota
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
ORCID
Takemoto, Mami
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Hishikawa, Nozomi
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Ohta, Yasuyuki
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Abe, Koji
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Abstract | The therapeutic effect of in vivo direct reprogramming on ischemic stroke has not been evaluated. In the present study, a retroviral solution (1.5-2.0 × 107 /ul) of mock pMX-GFP (n = 13) or pMX-Ascl1/Sox2/NeuroD1 (ASN) (n = 14) was directly injected into the ipsilateral striatum and cortex 3 days after 30 min of transient cerebral ischemia. The reprogrammed cells first expressed neuronal progenitor marker Dcx 7 and 21 days after viral injection, then expressed mature neuronal marker NeuN. This was accompanied by morphological changes, including long processes and synapse-like structures, 49 days after viral injection. Meanwhile, therapeutic improvement was not detected both in clinical scores or infarct volume. The present study provides a future novel self-repair strategy for ischemic stroke with beneficial modifications of the inducer-suppressor balance.
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Published Date | 2019-07-29
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Publication Title |
Scientific Reports
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Volume | volume9
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Issue | issue1
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Publisher | NPG
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Start Page | 10956
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ISSN | 2045-2322
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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Copyright Holders | © The Author(s) 2019
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File Version | publisher
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Related Url | isVersionOf https://doi.org/10.1038/s41598-019-47482-0
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License | http://creativecommons.org/licenses/by/4.0/
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Citation | Yamashita, T., Shang, J., Nakano, Y. et al. In vivo direct reprogramming of glial linage to mature neurons after cerebral ischemia. Sci Rep 9, 10956 (2019) doi:10.1038/s41598-019-47482-0
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Funder Name |
Ministry of Education, Culture, Sports, Science and Technology
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助成番号 | 17H04196
17K10827
15K15527
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