ID | 54772 |
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Author |
Itano, Satsuki
Graduate School of Environmental and life Science, Okayama University
Maeda, Megumi
Graduate School of Environmental and life Science, Okayama University
Kaken ID
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Md. Ziaur Rahman
Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Bangladesh Atomic Energy Commission
Kimura, Yoshinobu
Graduate School of Environmental and life Science, Okayama University
Kaken ID
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Abstract | We have identified, and purified to homogeneity, a high molecular weight Ginkgo biloba α-fucosidase (α-fucosidase Gb, 120 kDa estimated by SDS‒PAGE) with activity against α-fucosylated oligosaccharides. When a Lewis a epitope-containing N-glycan was used as a substrate, α-fucosidase Gb showed optimum activity at approximately pH 5.5, suggesting that it functions in acidic environments such as the vacuole. It remains uncertain, however, whether this Ginkgo α-fucosidase belongs to the GH29 family, since its N-terminal sequence could not be determined, probably due to a chemical modification. α-Fucosidase Gb showed substantial activity towards the α1,3-fucosyl linkage in Lacto-N-fucopentaose III and an α1,4-fucosyl linkage in the Lewis a epitope found in plant complex type N-glycans, indicating an involvement in the degradation process of α-fucosylated oligosaccharides or N-glycoproteins.
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Abstract Alternative | 銀杏種子から高分子量 (SDS-PAGE で120 kDa) を有し,α-フコース含有オリゴ糖に活性を示すα-フコシダーゼ(α-fucosidase Gb)を均一に精製した.ルイス a エピトープ含有 N-グリカンを基質とした場合,α-fucosidase Gb の至適 pH は 5.5 付近であることから,本酵素は液胞のような酸性環境で機能していることが示唆された.N‒末端アミノ酸配列が化学修飾のため同定できなかったため,本酵素が GH29 ファミリーに属するかどうかは不明である.α-Fucosidase Gb は,Lacto-N-fucopentaose IIIの α1,3-フコース残基やルイス a エピトープ含有の植物複合型N-グリカンのα1,4-フコース残基を加水分解することから,α-フコース含有オリゴ糖やN 型糖タンパク質の分解プロセスに関与することが示唆された.
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Keywords | α-fucosidase
plant N-glycan
N-glycan degradation
Ginkgo biloba
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Note | 原著論文 (Original paper)
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Published Date | 2017-02-01
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Publication Title |
Scientific Reports of the Faculty of Agriculture, Okayama University
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Volume | volume106
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Publisher | 岡山大学農学部
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Start Page | 5
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End Page | 12
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ISSN | 2186-7755
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Content Type |
Departmental Bulletin Paper
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language |
English
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File Version | publisher
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Refereed |
False
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Eprints Journal Name | srfa
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