ID | 40253 |
JaLCDOI | |
Sort Key | 4
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Title Alternative | Studies on Paper-analysis in the Field of Balneology. (3) The Changes of the Serum Protein Fractions and Lipoprotein Fractions Following the Bathing in Radioactive Hot Spring
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FullText URL | |
Author |
Ishibashi, Maruo
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Abstract | I. Some fundamental conditions and procedures on filter-paper electrophoresis were investigated. The electrophoretic apparatus of Natsume Seisakusho and Toyo No. 51 filter-paper were used. Electrophoresis was carried out under the current of 0.25 mA/cm. in 300 Volt during 5 hours, using veronal buffer pH 8.6 (μ=0.05). In
measuring of serum protein fractions, the adequate volume of the materials loaded is in the range of 0.005~0.01 ml. and in the case of lipoprotein, 0.02 ml. of serum is used. Paper-strip was stained with bromophenol blue or sudan black B, then making it transparent with heat-solved paraffin and photometered by Nataume's
densitometer. The reproducibility of the above-mentioned method with a confidence of 99% was as follows:
Serum protein fractions
Albumin 53.4±1.8%
α(1)-Globulin 3.3±0.3
α(2)-Globulin 6.0±0.5
β-Globulin 12.2±0.7
γ-Globulin 25.1±1.1
Serum lipoprotein fractions
α 20.6±2.6%
β+γ 79.4±2.6
II. Changes in serum protein fractions and lipoprotein fractions following the radioactive hot spring bathing were as follows: 1). In rabbits, the albumin-fraction of serum protein increased after a series of baths in radioactive hot spring, and the β- and γ-globulin-fractions decreased. 2). By cholesterol-feeding, the albumin-fraction of serum protein of rabbits decreased and the β- and γ-globulin-fractions increased significantly but when a series of baths was carried out in cholesterol-fed rabbits the decrease in the albumin-fraction was slighter than the former. 3). α-globulin-fraction of serum lipoprotein increased after single bathing in
radioactive hot spring and (β+γ) -globulin-fractions showed an inverse change, but the changes of lipoprotein-fractions following a series of baths were not remarkable. 4). By cholesterol-feeding, the ratio of lipoprotein-fractions (β+γ/α) increased, but the ratio tended to decrease following a series of baths in radioactive hot spring.
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Note | 正誤表あり
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Publication Title |
岡山大学温泉研究所報告
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Published Date | 1959-01
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Volume | volume24
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Publisher | 岡山大学温泉研究所
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Publisher Alternative | Institute for Thermal Spring Research, Okayama University
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Start Page | 54
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End Page | 79
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ISSN | 0369-7142
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NCID | AN00032853
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Content Type |
Departmental Bulletin Paper
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Related Url | http://ousar.lib.okayama-u.ac.jp/metadata/40250
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OAI-PMH Set |
岡山大学
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language |
Japanese
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File Version | publisher
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NAID | |
Eprints Journal Name | mmc
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