ID | 17390 |
Eprint ID | 17390
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FullText URL | |
Title Alternative | Studies on the cornin extracted from liver 2. Purification of the rat liver microsomal cornin and its chemical properties
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Author |
Doi, Akitaka
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Abstract | A curude cornin extracted from rat liver microsomes and the chemical properties of the purified cornin (MF1) were investigated. 1. Crude rat liver microsomal cornin (RLMC) inhibits the growth of L-929 cells. MF1 is purified about 4 fold from RLMC by gel filtration through Sephadex G-50 column. 2. The concentration of MFI that inhibits the growth of L cells to 50% is 270 μg/ml and this specific activity is the highest of the cornin fractions reported up to date. 3. The content of acidic amino acids is more than 23% but that of histidine and sulfurcontaining amino acids is very low in MFl. 4. MFl shows the maximum ultraviolet absorption at 276nm and its main substance is a kind of protein, and its inhibitory effect on the cell growth diminishes by protease treatment. 5. MFl is a single peak both by polyacrylamide gel electrophoresis and by thin layer gel filtration and the molecular weight is calculated about 26,000. 6. MFl is separated in two bands by SDS-polyacrilamide gel electrophoresis and the molecular weight is estimated to be 11,000 and 15,000 respectively.
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Published Date | 1978-08
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Publication Title |
岡山医学会雑誌
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Publication Title Alternative | Journal of Okayama Medical Association
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Volume | volume90
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Issue | issue7-8
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Publisher | 岡山医学会
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Publisher Alternative | Okayama Medical Association
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Start Page | 841
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End Page | 849
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ISSN | 0030-1558
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NCID | AN00032489
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Content Type |
Journal Article
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Official Url | https://www.jstage.jst.go.jp/article/joma1947/90/7-8/90_7-8_841/_article/-char/ja/
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Related Url | http://www.okayama-u.ac.jp/user/oma/
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language |
Japanese
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Copyright Holders | 岡山医学会
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File Version | publisher
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Refereed |
True
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Eprints Journal Name | joma
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