ID | 17823 |
Eprint ID | 17823
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FullText URL | |
Title Alternative | DNA synthesis inhibiting factors of muscle-cornin and cornea-cornin
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Author |
Nisida, Isamu
Murakami, Tetsuhide
Makiyama, Masao
Ohno, Naofumi
Nakarai, Akihide
Kakiuchi, Ichiro
Ikemune, Hironori
Ohtsuki, Hisashi
Chikata, Yoshiko
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Abstract | An attempt was made to purify the active principle (s) of cornea-and muscle-cornin, inhibiting the DNA synthesis in cultured mammalian cells. Each of the two kinds of cornin was separated into the dialysable (D) and the undialysable (U) fraction, which were further fractionated by the use of Sephadex gel. The inhibitory rate of these fractions was indicated by percent of control estimating the incorporation of (3)H-thymidine into cells or DNA fractions of cultured rat liver cells, line RLN-10, during the incubation period of 6 hours at 37℃ in the presence of respective fractions at the final concentration of 0.5 percent in the culture medium. As a result, the highest activity was shown in the D-Ⅱ fraction of muscle-cornin, which corresponded to the elution peak of the inorganic phosphate, and in the D fraction of cornea-cornin, The activity, however, was also shown in several other fractions. These data suggest that the inhibitory effect of cornin on the DNA synthesis in mammalian cells is due to combined effects of several factors present in cornin.
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Published Date | 1972-06-30
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Publication Title |
岡山医学会雑誌
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Publication Title Alternative | Journal of Okayama Medical Association
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Volume | volume84
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Issue | issue5-6
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Publisher | 岡山医学会
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Publisher Alternative | Okayama Medical Association
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Start Page | 119
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End Page | 125
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ISSN | 0030-1558
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NCID | AN00032489
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Content Type |
Journal Article
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Official Url | https://www.jstage.jst.go.jp/article/joma1947/84/5-6/84_5-6_119/_article/-char/ja/
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Related Url | http://www.okayama-u.ac.jp/user/oma/
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language |
Japanese
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Copyright Holders | 岡山医学会
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File Version | publisher
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Refereed |
True
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Eprints Journal Name | joma
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