ID | 14234 |
Eprint ID | 14234
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FullText URL | |
Title Alternative | Persistent herpes simplex virus type 2 infection in a hamster kidney cell line with a characteristic to grow at a high culture temperature
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Author |
Kitamura, Tetsuro
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Abstract | A subline of the baby hamster kidney cell line (BHK-21), which was adapted to grow at 41℃ and named BHK-21H, was used to examine the effect of pre-treatment of host cells with a high culture temperature on the growth of herpes simplex virus (HSV). BHK-21H cells were infected with HSV type 2 (HSV-2) at 41℃ and maintained for 7 days at the same temperature. When these cells were further cultivated after shift-down to 36℃, persistent infections were established. These infections were characterized by a carrier culture state ; the infected cultures were composed of infected and non-infected cells, and cell destruction and regrowth of cells caused a balanced state of culture. Two to 3 months after the establishment of the persistent infections, virus production in the cultures stopped spontaneously and colonial regrowth of uninfected cells was observed. A subline (BHK-21R) which was cured of the persistent infection was obtained. Cells of this subline showed resistance to reinfection of HSV. Persistent HSV-2 Infection was also established by pre-treatment of BHK-21H cells at 41℃ before virus infection and maintenance of the infected cultures at 36℃. These findings indicate that establishment of the persistent HSV-2 infection is mainly attributable to reduced permissiveness of BHK-21H cells for virus growth caused after heat treatment of the cells.
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Keywords | herpes simplex virus
persistent infection
supraoptimal temperature
carrier culture state
permissiveness
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Note | 原著
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Published Date | 1991-08
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Publication Title |
岡山医学会雑誌
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Publication Title Alternative | Journal of Okayama Medical Association
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Volume | volume103
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Issue | issue7-8
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Publisher | 岡山医学会
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Publisher Alternative | Okayama Medical Association
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Start Page | 859
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End Page | 867
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ISSN | 0030-1558
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NCID | AN00032489
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Content Type |
Journal Article
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Official Url | https://www.jstage.jst.go.jp/article/joma1947/103/7-8/103_7-8_859/_article/-char/ja/
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Related Url | http://www.okayama-u.ac.jp/user/oma/index.html
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language |
Japanese
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Copyright Holders | 岡山医学会
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File Version | publisher
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Refereed |
True
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Eprints Journal Name | joma
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