| ID | 14207 |
| Eprint ID | 14207
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| FullText URL | |
| Title Alternative | Medico-legal studies on the sex-identification of biological materials by DNA analysis
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| Author |
Nakai, Miyoko
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| Abstract | Sex identification of human blood, bloodstains, saliva, hair roots and bone marrow was conducted by DNA analysis. The effects of various contaminants in samples on sex identification were studied, using 32-week-old contaminated bloodstains. The effect of heat or decomposition on sex identification was examined by using the blood obtained from autopsy-cases. A 2.12 kb band with male DNA, digested with Hae Ⅲ and electrophoresed, was observed by Southern hybridization using a human Y chromosome-specific probe, while a faint 2.00 kb band with female DNA was also sometimes detected. The male 2.12 kb band was detected even in 5-year-old bloodstains. The 2.12 kb band was also detected frequently in contaminated bloodstains as old as 32 weeks. The band was observed by Southern hybridization analysis in decomposed blood as old as 7 days, while it could not be detected in heated and decomposed blood after 3 days.
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| Keywords | sex identification
deoxyribonucleic acid (DNA)
forensic science
bloodstains
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| Note | 原著
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| Published Date | 1991-08
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| Publication Title |
岡山医学会雑誌
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| Publication Title Alternative | Journal of Okayama Medical Association
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| Volume | volume103
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| Issue | issue7-8
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| Publisher | 岡山医学会
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| Publisher Alternative | Okayama Medical Association
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| Start Page | 823
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| End Page | 831
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| ISSN | 0030-1558
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| NCID | AN00032489
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| Content Type |
Journal Article
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| Official Url | https://www.jstage.jst.go.jp/article/joma1947/103/7-8/103_7-8_823/_article/-char/ja/
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| Related Url | http://www.okayama-u.ac.jp/user/oma/index.html
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| language |
Japanese
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| Copyright Holders | 岡山医学会
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| File Version | publisher
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| Refereed |
True
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| Eprints Journal Name | joma
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