| JaLCDOI | 10.18926/AMO/30742 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Tajiri, Takuma| Tate, Genshu| Iwaku, Takeshi| Takeyama, Nobuyuki| Fusama, Shigeyoshi| Sato, Shuichi| Kunimura, Toshiaki| Mitsuya, Toshiyuki| Morohoshi, Toshio| |
| Abstract | Right pleural effusion was diagnosed in a 36-year-old woman with right upper quadrant pain and fever. Enhanced pelvic computed tomography performed because of irregular genital bleeding revealed the pelvic inflammatory disease. Upon further questioning, the patient confirmed that she had recently undergone therapy for Chlamydia trachomatis infection. Therefore she was given an injection of tetracycline because we suspected Fitz-Hugh-Curtis syndrome (FHCS), a pelvic inflammatory disease characterized by perihepatitis associated with chlamydial infection. A remarkable clinical response to antibiotics was noted. The right upper quadrant pain was due to perihepatitis, and the final diagnosis was FHCS. Right pleural effusion may be caused by inflammation of the diaphragm associated with perihepatitis. Once chlamydial infection reaches the subphrenic liver, conditions in the closed space between the liver and diaphragm due to inflammatory adhesion may be conductive to chlamydial proliferation. The possibility of FHCS should be considered in patients and carefully distinguished from other abdominal diseases. |
| Keywords | perihepatitis right pleural eff usion Fitz-Hugh-Curtis syndrome chlamydial infection pelvic inflammatory disease |
| Amo Type | Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2006-10 |
| Volume | volume60 |
| Issue | issue5 |
| Publisher | Okayama University Medical School |
| Start Page | 289 |
| End Page | 294 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 17072375 |
| Web of Science KeyUT | 000241509000005 |
| JaLCDOI | 10.18926/AMO/30741 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Nagasaka, Takeshi| Goel, Ajay| Matsubara, Nagahide| Tanaka, Noriaki| |
| Abstract | Aberrant promoter methylation, an 'epigenetic' form of genomic instability that leads to transcriptional silencing of tumor suppressor genes, is increasingly being recognized as a crucial component in the evolution of human cancers. With our limited knowledge of the molecular basis and timing of the initiation of altered methylation events in the stepwise progression of cancers, the biggest challenge we currently face is to identify novel biomarkers and technologies for the timely screening of patients carrying such alterations. One such strategy would be to develop tests for the detection of fecal DNA methylation patterns that will improve the sensitivity of noninvasive screening tests for colorectal neoplasia, and moreover, will decrease both mortality and the incremental costs of treating colorectal cancers. |
| Keywords | fecal DNA colorectal cancer methylation epigenetics |
| Amo Type | Review |
| Publication Title | Acta Medica Okayama |
| Published Date | 2006-10 |
| Volume | volume60 |
| Issue | issue5 |
| Publisher | Okayama University Medical School |
| Start Page | 249 |
| End Page | 256 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 17072371 |
| Web of Science KeyUT | 000241509000001 |
| JaLCDOI | 10.18926/AMO/30740 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Une, Tomoka| Yokoyama, Yuji| Ninomiya, Shinsuke| Shinozuka, Masako| Maruyama, Hidehiko| Morishima, Tsuneo| |
| Abstract | Some marker chromosomes and chromosome rearrangements are difficult to identify using G-bands by Giemsa staining after trypsin treatment (G-banding) alone. Molecular cytogenetic techniques, such as spectral karyotyping (SKY) and fluorescence in situ hybridization (FISH), can help to detect chromosomal aberrations precisely. We analyzed the karyotypes in 6 cases of multiple congenital abnormalities and 1 case of spontaneous abortion (case 2). Three cases (cases 1, 6, and 7) had marker chromosomes, and 4 cases (cases 2-5) had chromosomal rearrangements. The karyotypes in cases 1, 2, and 3 were determined using FISH with probes based on the clinical findings and family histories. Spectral karyotyping (SKY) analysis in cases 4-7 showed that this method is useful and saves time. The combination of SKY and FISH analyses defi ned the range of the ring chromosome in case 7. We demonstrated that a combination of G-banding, FISH, and SKY can be applied effectively to the investigation of chromosomal rearrangement and to the detection of marker chromosome origins. We suggest the use of these methods for prenatal diagnosis, in which the inherent time limitations are particularly important. |
| Keywords | spectral karyotyping fluorescence in situ hybridization molecular cytogenetics marker chromosome chromosome rearrangement |
| Amo Type | Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2006-10 |
| Volume | volume60 |
| Issue | issue5 |
| Publisher | Okayama University Medical School |
| Start Page | 279 |
| End Page | 287 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 17072374 |
| Web of Science KeyUT | 000241509000004 |
| JaLCDOI | 10.18926/AMO/30739 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Aiga, Ayako| Asaumi, Koji| Lee, You Jin| Kadota, Hiroaki| Mitani, Shigeru| Ozaki, Toshifumi| Takigawa, Masaharu| |
| Abstract | The localization and expression of neurotrophins and their receptors during distraction osteogenesis was investigated in 72 male rat femurs (11 weeks old) to further clarify the concurrence of cellular and molecular events of new bone formation. After osteotomy, a 7-day lag phase was followed by distraction at the rate of 0.25 mm/12 h for 21 days (distraction phase), and a 7-day consolidation phase. The localization of neurotrophins (NGF, BDNF and NT-3) and their receptors tropomyosinrelated kinases (TRKA, TRKB and TRKC) by immunostaining showed positive staining in bone forming cells in each stage, although the presence and staining intensity varied by cell type and phase. The expressions of NGF, BDNF and NT-3 by real-time polymerase chain reaction (real-time PCR) showed that the peak of the mRNA expression of NGF occurred 10 days after distraction. NT-3 increased during bone extension, but decreased when distraction stopped. In contrast, BDNF continued to increase gradually throughout the distraction and consolidation phases. These findings suggest that neurotrophins and their receptors may play different roles in endochondral and intramembranous ossification in distraction osteogenesis. The tension stress caused by distraction may stimulate the expression of neurotrophins and their receptors, and promote osteogenesis. |
| Keywords | neurotrophin Trk distraction osteogenesis mechanical stress |
| Amo Type | Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2006-10 |
| Volume | volume60 |
| Issue | issue5 |
| Publisher | Okayama University Medical School |
| Start Page | 267 |
| End Page | 277 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 17072373 |
| Web of Science KeyUT | 000241509000003 |
| JaLCDOI | 10.18926/AMO/30738 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Ohtani, Yuu| Aoe, Motoi| Hara, Fumikata| Tao, Hiroyuki| Koshimune, Ryuichiro| Hirami, Yuuji| Hanabata, Tetsuro| Shimizu, Nobuyoshi| |
| Abstract | To investigate the suppressive effect of human recombinant TIMP-1 (rh-TIMP-1) on tumor proliferation using an in vivo xenograft system, HT29 was suspended in 0.1 ml phosphate buffered saline (PBS) and then subcutaneously injected in the back of female mice (BALB/C nu/nu). The mice were divided into 2 groups an and the tumor diameter was measured after rh-TIMP-1 (2 mg/kg) (rh-TIMP-1 group) or PBS (control group) was administered injections according to the following schedules. Schedule 1 : Beginning 2 weeks after the subcutaneous injection of HT29, an intraperitoneal injection of rh-TIMP-1 or PBS were performed twice a day (every 12 h) for 14 consecutive days. Schedule 2 : Beginning 1 week after the subcutaneous injection of HT29, an intraperitoneal injection was performed twice a day for 14 consecutive days. Schedule 3 : Intraperitoneal injections were started simultaneously with the subcutaneous injection of HT29, and then performed twice a day for 21 consecutive days. The mice were sacrificed and the tumors extirpated for immunohistochemical investigation. In addition, gelatin zymography and a cell proliferation assay were performed. With Schedule 1, the changes in the tumor diameter in the rh-TIMP-1 group followed the same course as those in the control group, and no suppressive effect on tumor proliferation was observed. However, with Schedule 3, a remarkable suppressive effect was observed throughout the treatment period. In immunostaining, more cases negative for MMP-9 were observed in the rh-TIMP-1 group than in the control group. Cases negative for CD34 were significantly more observed in the rh-TIMP-1 group than in the control group with Schedule 3. All of the results were obtained through the suppressive effect of rh-TIMP-1 on angiogenesis. |
| Keywords | MMP-2 MMP-9 TIMP-1 molecular targeting therapy angiogenesis |
| Amo Type | Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2006-10 |
| Volume | volume60 |
| Issue | issue5 |
| Publisher | Okayama University Medical School |
| Start Page | 257 |
| End Page | 266 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 17072372 |
| Web of Science KeyUT | 000241509000002 |
| JaLCDOI | 10.18926/AMO/30737 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Suzaki, Noriyuki| Hiraki, Akio| Takigawa, Nagio| Ueoka, Hiroshi| Tanimoto, Yasushi| Kozuki, Toshiyuki| Tabata, Masahiro| Kanehiro, Arihiko| Kiura, Katsuyuki| Tanimoto, Mitsune| |
| Abstract | A 71-year-old Japanese man with adenocarcinoma of the lung developed interstitial pneumonia after treatment with paclitaxel. The patient had acute chills and fever on the fourth day after the second exposure to paclitaxel, rapidly got worse despite empiric therapies, and developed prolonged respiratory failure requiring mechanical ventilation. Four months later, he died of respiratory failure due to progression of both interstitial pneumonia and lung cancer. This is the first case developing fatal paclitaxel-induced pulmonary toxicity to date. Interstitial pneumonia should be considered one of the possible life-threatening complications during treatment with paclitaxel. |
| Keywords | paclitaxel adverse effect lung cancer interstitial pneumonia |
| Amo Type | Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2006-10 |
| Volume | volume60 |
| Issue | issue5 |
| Publisher | Okayama University Medical School |
| Start Page | 295 |
| End Page | 298 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 17072376 |
| Web of Science KeyUT | 000241509000006 |
