ID | 31688 |
JaLCDOI | |
FullText URL | |
Author |
Mouri, Hirokazu
Sakaguchi, Kohsaku
Sawayama, Tomoyuki
Senoh, Tomonori
Ohta, Takeyuki
Nishimura, Mamoru
Fujiwara, Akiko
Terao, Masako
Shiratori, Yasushi
Tsuji, Takao
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Abstract | Transforming growth factor-beta1 (TGF-beta1) exerts potent immunosuppressive effects. In this study, we investigated the potential role of TGF-beta1 produced by hepatocellular carcinoma (HCC) cell lines in immunosuppression mechanisms. Using the Mv1Lu cell-growth inhibition assay and an enzyme-linked immunosorbent assay (ELISA), we detected optimal levels of TGF-beta1 in the culture supernatants conditioned by the HCC cell lines PLC/PRF/5, Hep3B, and HepG2. To determine the biological activity of TGF-beta1 in the supernatants, we examined the effects of the culture supernatants on the production of interferon (IFN)-gamma induced during the culture of peripheral blood mononuclear cells (PBMCs) stimulated with interleukin (IL)-12. IFN-gamma production of IL-12-stimulated PBMCs in the 1:1 dilution of the acid-activated conditioned medium of PLC/PRF/5, Hep3B, and HepG2 reduced to 14.7 +/- 0.8, 17.3 +/- 9.0, and 35.9 +/- 14.6%, respectively, compared with the value in the culture with control medium (complete culture medium). These results suggest that HCC cells producing TGF-beta1 may reduce the generation or activation of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells, and thus could enhance their ability to escape immune-mediated surveillance. |
Keywords | hepatocellular carcinoma
immunosuppression
transforming growth factor-?1 (TGF-?1)
interleukin-12
interferon-? (IFN-?)
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Amo Type | Article
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Publication Title |
Acta Medica Okayama
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Published Date | 2002-12
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Volume | volume56
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Issue | issue6
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Publisher | Okayama University Medical School
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Start Page | 309
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End Page | 315
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ISSN | 0386-300X
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NCID | AA00508441
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Content Type |
Journal Article
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language |
English
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File Version | publisher
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Refereed |
True
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PubMed ID | |
Web of Science KeyUT |