JaLCDOI 10.18926/AMO/31324
FullText URL fulltext.pdf
Author Koide, Norio| Shinji, Toshiyuki| Okada, Ken| Mizushima, Jun| Matsuda, Nobuyoshi| Sunami, Hiroshi|
Abstract The aim of the present study was to find the cause of inter-laboratory differences in laboratory test data and to examine whether control assessment helps to reduce inter-laboratory differences. Blood and serum samples of one healthy subject and one subject with liver cirrhosis were analyzed by 11 laboratories in the Okayama City area. No differences were found in the assay units of 26 tests surveyed. However, considerable differences were observed in test data, reference interval, and clinical level (CL), though most laboratories pointed out that the test data for the normal subject was within the reference intervals and those for the patient with liver cirrhosis showed abnormalities in tests for liver function. The difference in reference intervals was serious in the tests of direct bilirubin (D-Bil), thymol turbidity test (TTT), alkaline phosphatase (ALP), gamma-glutamyltranspeptidase (GGTP) and choline sterase. Marked differences in CLs were found in the tests of D-Bil, TTT, ALP, GGTP, creatine phosphokinase, amylase, heavy density lipoprotein cholesterol and white blood cell count. However, three hepatologists independently suggested that such inter-laboratory differences would not seriously affect a clinical decision on the disease status of the cirrhotic patient. Most tests that showed a trend error in a recent quality control survey appeared to have the same trend in the present study. These results indicate that inter-laboratory differences occur at various levels and control assessment are helpful in establishing, and therefore reducing, the level of inter-laboratory differences.
Keywords inter-labpratory differrence liver cirrhosis referrence interval clinical level control survey
Amo Type Article
Published Date 1998-10
Publication Title Acta Medica Okayama
Volume volume52
Issue issue5
Publisher Okayama University Medical School
Start Page 261
End Page 270
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9810436
Web of Sience KeyUT 000076694300005
JaLCDOI 10.18926/AMO/31323
FullText URL fulltext.pdf
Author Wakimoto, Masahiro| Masuoka, Noriyoshi| Nakano, Taku| Ubuka, Toshihiko|
Abstract <p>A new method for the determination of glutathione peroxidase activity in erythrocytes was developed. The present method was applied to the measurement of hydrogen peroxide removal rates by glutathione peroxidase in erythrocytes at 70 microM hydrogen peroxide under simulated in vivo conditions. The removal rates by glutathione peroxidase in mouse erythrocytes were twenty-times faster than those in human ones and were 5.2 mumol/sec/g of Hb. The removal rates in acatalasemic mouse erythrocytes indicate that glutathione peroxidase is the main means of hydrogen peroxide removal in acatalasemic mouse erythrocytes. Based on these results, we concluded that glutathione peroxidase in mouse erythrocytes had sufficient ability to remove hydrogen peroxide at even relatively high concentrations. This may be one of the reasons why acatalasemic mice suffer no health problems while Japanese acatalasemic patients suffer from Takahara disease when infected with hydrogen peroxide-generating bacteria.</p>
Keywords glutathione peroxidase erythrocyte hydrogen peroxide acatalasemic mouse Takahara disease
Amo Type Article
Published Date 1998-10
Publication Title Acta Medica Okayama
Volume volume52
Issue issue5
Publisher Okayama University Medical School
Start Page 233
End Page 237
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9810432
Web of Science KeyUT 000076694300001
JaLCDOI 10.18926/AMO/31322
FullText URL fulltext.pdf
Author Takeuchi, Hitoshi| Tamura, Ryuuji| Baba, Takako| Kawashima, Takeshi| Fukazawa, Takuya| Yunoki, Yasuhiro| Tanakaya, Koji| Yasui, Yoshimasa| Konaga, Eiji|
Abstract <p>Percutaneous microwave coagulation therapy (PMCT) is a new technique for the treatment of hepatocellular carcinoma (HCC). However, it is difficult to distinguish those lesions in which necrosis has been induced from the viable residual lesions during the procedure, because the margin of the tumor becomes unclear during PMCT. We determined the area of necrotic lesions during the procedure using color Doppler imaging. PMCT was performed on 10 patients (17 lesions) with recurrent HCC. The electrode of the microwave delivery system was moved around the tumor and the surrounding area until color mosaic images disappeared from the entire area of the tumor. The areas in which necrotic tissue was indicated by color Doppler imaging were later confirmed by other modalities such as angiography or contrast-enhanced computed tomography. This leads us to believe that real-time, effective evaluation of PMCT is possible with color Doppler imaging.</p>
Keywords microwave coagulation therapy color Doppler imaging hepatocellular carcinoma
Amo Type Article
Published Date 1998-10
Publication Title Acta Medica Okayama
Volume volume52
Issue issue5
Publisher Okayama University Medical School
Start Page 255
End Page 260
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9810435
Web of Science KeyUT 000076694300004
JaLCDOI 10.18926/AMO/31321
FullText URL fulltext.pdf
Author Takechi, Hirosuke| Tokuhiro, Akihiro|
Abstract <p>We studied the seated buttock pressure distribution in six paraplegic patients by means of computerized pressure mapping. They were all male and their age ranged from 18 to 48 years old. Their level of paralysis varied from Th5 to L1. Five kinds of wheelchair cushions were studied: an air cushion, a contour cushion, a polyurethane foam cushion, a Cubicushion (which is made of polyurethane foams) and a silicone gel cushion. A tactile sensor consisting of 2064 matrices was used for measuring the buttock pressure distribution and the data was analyzed on a personal computer. Peak pressures measured for each cushion were as follows (in descending order): the Cubicushion, the polyurethane foam cushion, the contour cushion, the silicone gel cushion, and the air cushion. The areas of total contact measured for each cushion were as follows (in descending order): the air cushion, the silicone gel cushion, the polyurethane foam cushion, the contour cushion and the Cubicushion. Based on these findings, we conclude that the most advantageous cushion is the air cushion or the silicone gel cushion. Likewise, we conclude that the Cubicushion is not practical for pressure sore prevention.</p>
Keywords Paraplegia wheelchair cushion pressure distribution
Amo Type Article
Published Date 1998-10
Publication Title Acta Medica Okayama
Volume volume52
Issue issue5
Publisher Okayama University Medical School
Start Page 245
End Page 254
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9810434
Web of Science KeyUT 000076694300003
JaLCDOI 10.18926/AMO/31320
FullText URL fulltext.pdf
Author Murakami, Takuro| Mabuchi, Masaru| Giuvarasteanu, Lleana| Kikuta, Akio| Ohtsuka, Aiji|
Abstract <p>Some rare anomalies of the celiaco-mesenteric system were observed postmortem in a Japanese adult male: a) The left gastric, common hepatic, splenic and superior mesenteric arteries arose independently from the abdominal aorta. b) The anterior inferior pancreaticoduodenal artery of the superior mesenteric artery issued a hepatic artery which ascended along the anterior surface of the pancreas and gave off the right gastroepiploic, right gastric and cystic arteries. c) The common hepatic artery gave off an anastomosing branch to the superior mesenteric artery. d) The left gastric artery gave off the left accessory hepatic artery. e) The splenic artery issued the accessory middle colic artery. f) The left inferior phrenic artery gave off the esophageal branch. These anomalies are discussed in light of a typological system which we proposed in a previous paper for the celiaco-mesenteric system.</p>
Keywords arterial anomaly celiac trunk left gastric artery hepatic artery splenic artery intermesenteric anastomosis
Amo Type Article
Published Date 1998-10
Publication Title Acta Medica Okayama
Volume volume52
Issue issue5
Publisher Okayama University Medical School
Start Page 239
End Page 244
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9810433
Web of Science KeyUT 000076694300002
JaLCDOI 10.18926/AMO/31319
FullText URL fulltext.pdf
Author Matsuo, Toshihiko|
Abstract <p>To study the expression of the amiloride-sensitive sodium channel, a putative mechano-receptor in the rat eye, reverse transcriptase-polymerase chain reaction and in situ hybridization were done. The gene for the alpha subunit of the amiloride-sensitive sodium channel was shown by polymerase chain reaction to be expressed in mRNA isolated from the whole eye tissue. In situ hybridization demonstrated that the gene was expressed in basal layers of the corneal and conjunctival epithelium, ciliary epithelial cells, lens epithelial cells at the equator, retinal and iris pigment epithelial cells, ganglion cells and cells in the inner and outer nuclear layers of the retina. The results suggest that the amiloride-sensitive sodium channel plays a role in maintaining sodium balance as well as in possible mechanosensation in these ocular tissues.</p>
Keywords amiloride-sensitive sodium channel eye insitu hybridzation mechanosensation polymerase chain reaction
Amo Type Article
Published Date 1998-10
Publication Title Acta Medica Okayama
Volume volume52
Issue issue5
Publisher Okayama University Medical School
Start Page 279
End Page 283
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9810438
Web of Science KeyUT 000076694300007
JaLCDOI 10.18926/AMO/31318
FullText URL fulltext.pdf
Author Nagata, Hiroshi| Ohta, Takeo| Aoyama, Hideyasu|
Abstract <p>Nursing practitioners' perception of inpatients' anxiety, self-esteem, purpose-in-life and health locus of control.</p>
Keywords inpatient's mental health anxiety self-es-teem purpose-in-life health locus of control
Amo Type Article
Published Date 1998-10
Publication Title Acta Medica Okayama
Volume volume52
Issue issue5
Publisher Okayama University Medical School
Start Page 271
End Page 278
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9810437
Web of Science KeyUT 000076694300006