ID | 30401 |
JaLCDOI | |
FullText URL | |
Author |
Ogulener, Nuran
Larabal, Eda
Baysal, Firuz
Dikmen, Atilla
|
Abstract | The possible role of nitric oxide (NO) and vasoactive intestinal polypeptide on isoprenaline-induced relaxation of the mouse longitudinal gastric fundal strips precontracted with 5.4 x 10(-7) M carbachol was investigated. Isoprenaline (5 x 10(-7) M, 10(-6) M and 5 x 10(-6) M) produced a concentration-dependent relaxations. NG-nitro L-arginine (10(-4) M) partly inhibited isoprenaline-induced relaxation. The inhibitory action of NG-nitro L-arginine was reversed by 4 x 10(-4) M L-arginine but not by 4 x 10(-4) M D-arginine. NG-nitro L-arginine (10(-4) M) did not affect the relaxation caused by sodium nitroprusside (10(-6) M). Vasoactive intestinal polypeptide antibody 7913 (1:160 dilution) partly inhibited isoprenaline-induced relaxation. This inhibition was greater on the response to the higher isoprenaline concentration (5 x 10(-6) M) than to the lower concentration (10(-6) M). The combination of vasoactive intestinal polypeptide antibody and NG-nitro L-arginine significantly enhanced the inhibition on 10(-6) M isoprenaline action. These results suggest that nitric oxide and vasoactive intestinal polypeptide may partly contribute to the relaxation induced by isoprenaline in the mouse gastric fundus precontracted with carbachol. |
Keywords | isoprenaline
N<sub>G<sub>-nitro L-arginine(L-NOARG)
L-arginine(L-ARG)
D-arginine(D-ARG)
vasoactive intestinal polypeptide (VIP) antibody 7913
isolated mouse gastric fundus
|
Amo Type | Article
|
Publication Title |
Acta Medica Okayama
|
Published Date | 1995-10
|
Volume | volume49
|
Issue | issue5
|
Publisher | Okayama University Medical School
|
Start Page | 231
|
End Page | 236
|
ISSN | 0386-300X
|
NCID | AA00508441
|
Content Type |
Journal Article
|
language |
English
|
File Version | publisher
|
Refereed |
True
|
PubMed ID | |
Web of Science KeyUT |