Acta Medica Okayama volume24 issue4

Epidemiological study on the prevalence of SMON III Ibara City, Yoshii Town and Yubara Town in Okayama Prefecture was conducted and the following results were obtained. "Epidemization precession" (Die Prazession der Durchseuchung), described by Rudder which shows that ages common to this disease is shifting to younger generation as incidence rate increases, in the endemic regions was recognized in Ibara City and Yubara Town. The mode of the distribution of the interval between the primary and secondary patients in a family suggests that it corresponds to the incubation period. Distribution of the patients to the size of family is not adaptable to binomial model, but adaptable to chain binomial model, and the intra-household transmitted rate is 5 per cent, and the true intra-household transmitted rate is about two times as high as extra-household transmitted rate by the modified chain binomial model. Intra-hospital incidence among SMON patients and non-SMON patients as well as medical workers were observed at the time when the number of new patients increased in a hospital. In SMON patients, the rate of appendectomized ones was higher than that of non-SMON patients, suggesting that gastrointestinal tract has some relationship to the development of SMON disease.

For the purpose to reveal the substance as the source of offensive odor of the fish from the sea facing petroleum and petrochemical industries, analyses have been made on the sea water, industrial wastes, offensive odor fish meat and the eels kept in the sea or industrial waste for a certain period, by means of gas chromatography, infrared, and ultraviolet absorption spectrophotometries and mass-spectrometry. Observations revealed toluene as a possible main source responsible for the bad smelling of the fish. Finally, by analysing the meat extract of the fish kept in the sea water containing toluene and by the same smell as that of the offensive odor fish from the off-shore of the industries, it has been definitely confirmed that toluene is the very substance that imparts the offensive odor to fish. The activated sludge process proved to be an effective method to remove toluene from the industrial wastes. A discussion was made on the aliphatic carbohydrate as the possible source of the offensive odor of fish.

Die Autoren haben 48 Patienten mit chronischer Koronarinsuffizienz mit Nitrolingual Retard Kapseln bettlagerig oder ambulant behandelt. Nach ihren Ergebnissen kann man sagen, dass Nitroglycerin in einer Langzeitform als Nitrolingual Retard Kapsel den modernen Erfordenissen der Therapie der Koronarinsufflzienz entspricht, da damit iiber einem langeren Zeitraum hinweg gleichmassig Blutspiegelwerte erzielt werden konnen. So hat man einen guten therapeutischen Erfolg, dazu einen Riickgang der Anfallhaufigkeit und eine erhohte Belastbarkeit erreicht. Der Wirkstoff hat iiber einem Zeitraum von 10-12 Stunden sich konti. nuirlich diffundiert bzw. klinisch gewirkt. Bei der Einnahme von Nitro. lingual Retard sind keine Kopfschmerzen aufgetreten. Die Vertraglichkeit des Praparateserwies sich als hut.

A non.specific esterase activity was demonstrated III the jejunum of rats by an azoindoxyl method. 1) Microvilli of the jejunal epithelial cells were remarkably stained in non-frozen specimens and feebly in frozen specimens. 2) The other cytoplasmic structures, i. e. mitochondria, endoplasmic reticulum, nuclear envelope, plasma membrane and multivesicular body showed a positive reaction product in frozen sections but not in non-frozen blocks.

A human fetal fibroblast strain, belonging to a group resistant to SV40 transformation, was transformed by SV40 through a multiple inoculation procedure. Two independently transformed cells were described in comparison with each other. The proportions of cells with the nuclei possessing V antigen were 2.9% at the 5th passage in one strain and 1.1 % at the 4th passage in another, and they declined gradually as frequent passages were repeated. The percentages of the transformed cells with V antigen-positive nuclei were, in both strains, quantitatively compatible with those of the cells with the nuclei full of virus particles in crystalline arrays, which were demonstrated by immunofluorescent studies and electron microscopy.

An electron microscopic observation was made on the DNA's extracted from purified HeLa cell nuclei, mitochondria, and the whole cell, and fractionated by ethidium bromide-cesium chloride density gradient method or sucrose density gradient method. Nuclear DNA presents mainly long linear DNA derived from fragmented chromosomal DNA. In addition to this, the existence of small circular DNA molecules measuring 0.32 -1.78 μ, was confirmed. Mitochondrial DNA was mainly circular DNA, which measured 4.87 μ in the mean value of the contour lengths in the highest frequency group, and small circular DNA molecules, measuring 0.3-1.01 μ in contour length, were also found in an extremely low frequency.

Two cases of multiple foreign bodies, i. e., duplex coins in the esophagus are reported. These foreign bodies were removed by peroral esophagoscopy successfully. Significance of roentgen-ray diagnosis is emphasized, and subtle and yet specific roentgenograms of duplex coins in the esophagus are illustrated.

Die stark eisenhaltigen Praparate Ferrlecit, Kobaltjerrlecit, Ferrojolsan, Ferrojolcamma und Irradian wurden im Siemens Unterrichtsreaktor SUR 100 des Instituts fur Kernenergetikder Universitat Struttgart und der Technischen Universitat, Berlin mit einem NeutronenfluB von ca. 5.l06n/cm2 sec bestrahlt. Es ollte dabei untersucht werden, wie stark eisenhaltige Praparate mit diesem Reaktor aktiviert werden konnen. Es wird festgestellt, daB Eisen praktisch kaum aktiviert wird. Die Ergebnisse zeigen, daB nur das Kobattjerrlecit und das Irradian starker durch Neutrosen aktiviert werden. Die hohere Aktivitat des Kobaltjerrlecit wird auf die darin enthaltenen Elemente Mangan, Kupfer und Kobalt zuruckgefuhrt, die mit thermischen Neutronen gut aktivierbar sind. Die hohere Aktivitat des Irradian geht hauptsachlich auf β-Zerfalle zuruck. Das Phosphorisotop P-32 tragt einen groβen Anteil dazu bei. De rβ-Untergrund des Irradian bei hohen Energien, der mit einer kleinen Halbwertszeitabklingt, ist durch keines der angefuhrten Elemente im Irradian erklarbar. Der Mn-56Peak im Spektrum ist gut erkennbar, durch den β-Untergrund wird er aber zu einer hoheren Energie hin verschoben.

By assuming a three-compartment model, kinetic analysis of peripheral hyroxine distributions in various organs was represented by the alues calculated on the basis of the disappearance curve of 131I-T4 radioactivity in the serum, time　dependent curve of radioactivity over the liver　and urinary excretion of 131I-T4 in attempts to clarify the kinetic distribution　of the thyroxine and the time　dependent pool size of thyroxine in each　compartment such as serum pool, liver pool, and the other pool,　As a result it has been demonstrated that pool size3 of tyroxine, in the serum, liver and the other pool are enlarged in　hyperthyroidism, while　they are decreased in　hypothyroidism in respective pools.　We have recognized the reduction in the values of the liver pool size　of 131I-thyroxine and 131I-thyroxine excretion into the bile, while the increase　of 131I-thyroxine excretion into the urine in the cases of chronic hepatitis　and cirrhosis of the liver, but the thyroxine concentration in the serum to remain within the normal level in liver diseases. As a result of the reduction　in the liver pool size of 131I-thyroxine and in its uptake into the liver,　the other pool size enlarges to compensate the reduction and the function of the other pool is elevated.

As a step in the elucidation of immunity of human cancer from the standpoint of homotransplantation immunity, we conducted mixed cultures of regional lymph node cells from C3H mouse isotransplanted with methylcholanthrene-induced sarcoma (MC-tumor) together with the primary culture MC-tumor cells, and observed the behaviors of these lymph node cells to the MC-tumor cells and compared the effects of these lymph node cells with lhose of normal mouse lymph node cells by counting the growth number of tumor cells and also by cinematography. As a result, it has been demonstrated that the regional lymph node cells from the mouse isotransplanted with the MC-tumor (2mm3 in size) acquire a strong antitumor activity by 14 days after the transplantation, but such antitumor activity diminishes and disappears in the terminal stage of cancer. When the number of these lymphocytes is increased, there can be observed some dosage effect, but no complete inhibition of the tumor growth can be attained. The cinematographic observations of these regional lymph node cells in the mixed culture with tumor cells reveal that lymphocytes of small to intermediate size aggregate onto the tumor cells and inhibit the movement of the latter.