Acta Medica Okayama volume21 issue2

In the immunofluorescent study it has been revealed that rabbit sera immunized with transformed cells induced by SV-40 DNA, produce circulating antibody capable of re:lcting with intranuclear antigens synthesized by SV-40 complyte virus transforming process, In addition, the result confirmed that SV-40 DNA replicates DNA-containing viruses in the host cell and that also the genome coding for the synthesis of SV-40 tumor antigen is resposible for viral DNA.

For the purpose of revealing the molecular organization of the mitochondrial membrane the authors attempted to clarify the fine structure of reduced coenzyme Q-cytochrome c reductase and also studied how the CoQH2-cyt. c reductase is arranged in the mitochondrial membrane by systematic analyses of fractions from the purification process of CoQH2-cyt. c reductase. 1. Purified CoQH2-cyt. c reductase contained high concentration of cyt. b (9.5 mμmoles per mg protein) and cyt. Cl (4.5 mμmoles per mg protein), and was almost free from cyt. c, a, flavoproteins, primary dehydrogenases and ATPase. The enzyme complex also showed a high specific activity (48 μmoles of cyt. c reduced per mg protein per min at 30°). 2. CoQH2-cyt. c reductase was composed of particles of about 120 Å in diameter with irregular form, some time exhibiting electron opaque cores. In the loose aggregates of the particles, the size of each particle was about 95 Å in diameter. 3. An intimate correlation was observed between the particles of CoQH2cyt. c reductase and those on the surface of the NADH-cyt. c reductase fraction. 4. Regular arrays of uniform particles (about 82 Å in diameter with a center to center distance of about 100 Å) were observed on the surface of the submitochondrial membrane (brown membrane) obtained from beef heart mitochondria by treatment with deoxycholate (0.1 mg / mg protein) and KCl (72 g/l). The correlation between these particles and CoQH2-cyt. c reductase was discussed.

As a link in the series of studies on tumor specific immunity an attempt was made to clarify specificity if any, in aggregation of sensitized lymph-node cells on target cell in vitro. For this purpose sensitized regional lymph-node cells from isologous CsH mouse transplanted with A cells derived from CaH mouse mammary cancer were incubated with M cells derived from mammary cancer of homologous Cb mouse and HeLa-Ss cells as with A cells. The results are briefly summarized in the following. These sensitized regional lymph-node cells (A-L) inhibited the proliferation of A cells and M cells in tissue culture. When the interaction between the sensitized lymph-node cells and the terget cells was pursued over a long period by cinematography, these lymph-node cells became attached to the target cell by 6-to 12-hour culture in aggregation of rosette form, and by 30 hours some of the target cells were seen to undergo lysis. However, when these sensitized lymph-node cells were cultured with heterologous HeLa-S3 cells (derived from human uterine cancer), no such phenomena were observed. In the case with untreated normal lymph-node cells (control) there could be hardly observed any inhibitory effect on target cells. When the number of the target cells on which the lymph-node cells became attached was counted along with lapse of time, it was more numerous in the case of A and M cells but only a few in the case of HeLa-S3 cells. It seems that most of the sensitized lymph-node cells that inhibit the growth of the target cells become attached and aggregated fairly specifically onto the target cells.

A case of arteriosclerotic retinopathy associated with retinal venous thrombosis was treated with Anginin and the following results obtained: 1) Visual acuity was improved from 0.03 to 0.7. 2) Retinal hemorrhages were absorbed and pipe-stem sheathing of the branch of retinal artery decrease, with white sheathing remaining partially. 3) It was therefore considered that the pipe-stem sheathing was decreased because Anginin removed venous spasm and improved the blood stream of the branch of the artery, and that the organic changes already established on the arterial wall would remain as white sheathing. 4) Anginin could not prevent retinal veins from changing into white lines. 5) Consequently the authors considered that Anginin may be a drug effectively used for retinal arteriosclerosis and retinal venous thrombosis associated therewith.