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ID 32991
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Author
Anokye-Danso, Frederick
Anyanful, Akwasi
Sakube, Yasuji
Kagawa, Hiroaki
Abstract

Gene regulation during development is an important biological activity that leads to synthesis of biomolecules at specific locations and specific times. The single tropomyosin gene of Caenorhabditis elegans, tmy-1/lev-11 produces four isoforms of protein: two from the external promoter and two from the internal promoter. We investigated the internal promoter of tropomyosin to identify sequences that regulate expression of tmy-1 in the pharynx and intestine. By promoter deletion of tmy-1 reporters as well as by database analyses, a 100 bp fragment was identified that contained binding sequences for a GATA factor, for a chicken CdxA homolog and for a forkhead factor. Both the forkhead and CdxA binding sequences contributed to pharyngeal and intestinal expression. In addition, the GATA site also influenced intestinal expression of tmy-1 reporter. We showed that ELT-2 and PHA-4 proteins interact directly with the GATA and forkhead binding sequences, respectively, in gel mobility shift assays. RNAi knockdown of elt-2 diminished tmy-1::gfp expression in the intestine. In contrast to RNAi knockdown of pha-4, expression of tmy-1::gfp in pha-4;smg-1 mutants was slightly weaker to that of the wild type. Ectopic expression of PHA-4 and ELT-2 by heat shock were sufficient to elicit widespread expression of tmy- 1::lacZ reporter in embryos. We found no indication of a synergistic relation between ELT-2 and PHA-4. Based on our data, PHA-4 and CdxA function as general transcription factors for pharyngeal and intestinal regulation of tmy-1. We present models by which ELT-2, PHA-4 and CdxA orchestrate expression from the internal promoter of tmy-1.

Keywords
Caenorhabditis elegans
tropomyosin
HNF-3/PHA-4
GATA/ELT-2
transcription
Note
Published with permission from the copyright holder.
This is a author's copy,as published in Journal of Molecular Biology , 2008, volume 379, issue 2, pp201-211.
Publisher URL: http://dx.doi.org/10.1016/j.jmb.2007.11.103
Copyright © 2008 Elsevier B. V. All rights reserved.
Published Date
2008-05-14
Publication Title
Journal of Molecular Biology
Volume
volume379
Issue
issue2
Content Type
Journal Article
language
英語
Refereed
True
DOI
Submission Path
biology_general/39