ID | 32990 |
FullText URL | |
Author |
Yoshihara, Ritsuko
Kadota, Yusuke
Kawai, Saeko
Goto, Tomomi
Zhong, Ming
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Abstract | A two-step culture system was used to investigate the role of chondroitin sulfate (CS) B, which is mitogenic to B cells, in differentiation of B cells. Mouse spleen B cells were incubated for 3 days with CSB in the presence of interleukin (IL)-4 and IL-5. After washing, the cells were replated at 10(5) viable cells/well and recultured without CSB in the presence of IL-4 and IL-5. CSB dose-dependently increased IgM production, the greatest enhancement being 450%. Dextran sulfate had a similar effect, whereas other glycosaminoglycans, CSA, CSC, heparin and hyaluronic acid, were marginally effective. Treatment of B cells with CSB resulted in increases in the number of IgM-secreting cells and numbers of CD138-positive cells and CD45R/B220-negative cells. CSB-induced IgM production was inhibited by the protein kinase C (PKC) inhibitor GF109203X but not by the phosphatidylinositol 3-kinase (P13K) inhibitor wortmannin. These results demonstrated that CSB promoted differentiation of B cells in the presence of IL-4 and IL-5 and suggested that PKC but not P13K is crucial for CSB-induced IgM production. |
Keywords | chondroitin sulfate B (CSB)
murine B cells
IgM
differentiation
CD138
protein kinase C
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Note | Published with permission from the copyright holder.
This is a author's copy,as Cellular Immunology, 2007 Vol.250 Issue.1-2 pp.14-23 . |
Published Date | 2008-06-30
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Publication Title |
Cellular Immunology
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Volume | volume250
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Issue | issue1-2
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Start Page | 14
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End Page | 23
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Content Type |
Journal Article
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language |
English
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OAI-PMH Set |
岡山大学
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File Version | author
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DOI | |
Web of Science KeyUT |