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ID 11835
Eprint ID
11835
FullText URL
Thumnail K003340.pdf 77.1 KB
Title Alternative
1型糖尿病に対する移植療法に向けたヒト膵β細胞株の樹立
Author
Narushima, Michiki
Abstract
A human pancreatic beta-cell line that is functionally equivalent to primary beta-cells has not been available. We established a reversibly immortalized human beta-cell clone (NAKT-15) by transfection of primary human beta-cells with a retroviral vector containing simian virus 40 large T-antigen (SV40T) and human telomerase reverse transcriptase (hTERT) cDNAs flanked by paired loxP recombination targets, which allow deletion of SV40T and TERT by Cre recombinase. Reverted NAKT-15 cells expressed beta-cell transcription factors (Isl-1, Pax 6, Nkx 6.1, Pdx-1), prohormone convertases 1/3 and 2, and secretory granule proteins, and secreted insulin in response to glucose, similar to normal human islets. Transplantation of NAKT-15 cells into streptozotocin-induced diabetic severe combined immunodeficiency mice resulted in perfect control of blood glucose within 2 weeks; mice remained normoglycemic for longer than 30 weeks. The establishment of this cell line is one step toward a potential cure of diabetes by transplantation.
Keywords
EMBRYONIC STEM-CELLS
INSULIN-PRODUCING CELLS
PANCREATIC-ISLET CELLS
IN-VITRO
GENE
MICE
ESTABLISHMENT
DIFFERENTIATION
IDENTIFICATION
HYPERGLYCEMIA
Note
http://dx.doi.org/10.1038/nbt1145
Publication Title
Content Type
Thesis or Dissertation
Grant Number
甲第3340号
Granted Date
2007-03-23
Thesis Type
博士(医学)
Grantor
岡山大学
Official Url
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16186810&dopt=Abstract
language
日本語
File Version
none
Refereed
Unknown