ID | 11797 |
Eprint ID | 11797
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FullText URL | |
Title Alternative | ヒト単球におけるCD14およびToll like receptor-4発現に対するシプロフロキサシンの効果
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Author |
Katsuno, Gotaro
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Abstract | CD14/toll-like receptor (TLR)-4 complex on monocytes/macrophages can bind lipopolysaccharide (LPS) and transduce the signals intracellularly. An antibacterial drug, ciprofloxacin (CIP), has been reported to modulate the inflammatory and immune responses. In the present study, we examined the effects of CIP on the LPS-induced activation of monocytes isolated from human peripheral blood mononuclear cells (PBMC). CIP suppressed the expression of CD14, TLR-4, intercellular adhesion molecule (ICAM)-1, B7.1, B7.2, and CD40 and the production of tumor necrosis factor (TNF)-alpha induced by LPS in monocytes. CIP induced the production of prostaglandin (PG)E2 and increased intracellular cyclic adenosine monophosphate (cAMP) levels. Cyclooxygenase (COX)-2 inhibitors, NS398 and indomethacin, reversed the effects of CIP on TNF-alpha production and reduced the levels of different surface antigens, whereas a protein kinase A (PKA) inhibitor, H89, did not. Therefore, CIP might regulate the TNF-alpha production induced by LPS by inhibiting the expression of LPS receptor complex, which seems to be mediated by COX-2 but not the cAMP/PKA pathway.
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Keywords | lipopolysaccharide
monocyte
ciprofloxacin
prostaglandin
CD14
toll-like receptor-4
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Published Date | 2007-03-23
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Publication Title | |
Content Type |
Thesis or Dissertation
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Grant Number | 甲第3335号
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Granted Date | 2007-03-23
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Thesis Type |
Doctor of Philosophy in Medical Science
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Grantor | 岡山大学
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Official Url | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16552356&dopt=Abstract
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language |
Japanese
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File Version | none
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Refereed |
Unknown
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