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ID 11493
Eprint ID
11493
FullText URL
Thumnail O004165.pdf 121 KB
Title Alternative
高分化大腸癌株HT29 in vivo治療モデルにおけるHuman Recombinant Tissue Inhibitor of Metalloproteinases-1(TIMP-1)の腫瘍増殖抑制効果の検討
Author
Ohtani, Yu
Abstract
To investigate the suppressive effect of human recombinant TIMP-1 (rh-TIMP-1) on tumor proliferation using an in vivo xenograft system, HT29 was suspended in 0.1 ml phosphate buffered saline (PBS) and then subcutaneously injected in the back of female mice (BALB/C nu/nu). The mice were divided into 2 groups an and the tumor diameter was measured after rh-TIMP-1 (2 mg/kg) (rh-TIMP-1 group) or PBS (control group) was administered injections according to the following schedules. Schedule 1 : Beginning 2 weeks after the subcutaneous injection of HT29, an intraperitoneal injection of rh-TIMP-1 or PBS were performed twice a day (every 12 h) for 14 consecutive days. Schedule 2 : Beginning 1 week after the subcutaneous injection of HT29, an intraperitoneal injection was performed twice a day for 14 consecutive days. Schedule 3 : Intraperitoneal injections were started simultaneously with the subcutaneous injection of HT29, and then performed twice a day for 21 consecutive days. The mice were sacrificed and the tumors extirpated for immunohistochemical investigation. In addition, gelatin zymography and a cell proliferation assay were performed. With Schedule 1, the changes in the tumor diameter in the rh-TIMP-1 group followed the same course as those in the control group, and no suppressive effect on tumor proliferation was observed. However, with Schedule 3, a remarkable suppressive effect was observed throughout the treatment period. In immunostaining, more cases negative for MMP-9 were observed in the rh-TIMP-1 group than in the control group. Cases negative for CD34 were significantly more observed in the rh-TIMP-1 group than in the control group with Schedule 3. All of the results were obtained through the suppressive effect of rh-TIMP-1 on angiogenesis.
Keywords
MMP-2
MMP-9
TIMP-1
molecular targeting therapy
angiogenesis
Note
PUBMED http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17072372&dopt=Abstract
Published Date
2007-03-23
Publication Title
Content Type
Thesis or Dissertation
Grant Number
乙第4165号
Granted Date
2007-03-23
Thesis Type
Doctor of Philosophy in Medical Science
Grantor
岡山大学
Official Url
http://escholarship.lib.okayama-u.ac.jp/cgi/viewcontent.cgi?article=1335&context=amo
language
日本語
File Version
none
Refereed
Unknown