ID 60847
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La, The Mon Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Tachibana, Hiromi Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Li, Shun-Ai Center for Innovative Clinical Medicine, Okayama University Hospital
Abe, Tadashi Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Seiriki, Sayaka Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Nagaoka, Hikaru Division of Malaria Research, Proteo-Science Center, Ehime University
Takashima, Eizo Division of Malaria Research, Proteo-Science Center, Ehime University
Takeda, Tetsuya Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Ogawa, Daisuke Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kaken ID
Makino, Shin-Ichi Department of Nephrology, Graduate School of Medicine, Chiba University
Asanuma, Katsuhiko Department of Nephrology, Graduate School of Medicine, Chiba University
Watanabe, Masami Center for Innovative Clinical Medicine, Okayama University Hospital Kaken ID publons researchmap
Tian, Xuefei Department of Internal Medicine, Section of Nephrology, Yale University School of Medicine
Ishibe, Shuta Department of Internal Medicine, Section of Nephrology, Yale University School of Medicine
Sakane, Ayuko Department of Biochemistry, Tokushima University Graduate School of Medical Sciences
Sasaki, Takuya Department of Biochemistry, Tokushima University Graduate School of Medical Sciences
Wada, Jun Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID Kaken ID publons researchmap
Takei, Kohji Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kaken ID publons researchmap
Yamada, Hiroshi Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kaken ID publons researchmap
Abstract
Dynamin 1 is a neuronal endocytic protein that participates in vesicle formation by scission of invaginated membranes. Dynamin 1 is also expressed in the kidney; however, its physiological significance to this organ remains unknown. Here, we show that dynamin 1 is crucial for microtubule organization and stabilization in glomerular podocytes. By immunofluorescence and immunoelectron microscopy, dynamin 1 was concentrated at microtubules at primary processes in rat podocytes. By immunofluorescence of differentiated mouse podocytes (MPCs), dynamin 1 was often colocalized with microtubule bundles, which radially arranged toward periphery of expanded podocyte. In dynamin 1-depleted MPCs by RNAi, alpha-tubulin showed a dispersed linear filament-like localization, and microtubule bundles were rarely observed. Furthermore, dynamin 1 depletion resulted in the formation of discontinuous, short acetylated alpha-tubulin fragments, and the decrease of microtubule-rich protrusions. Dynamins 1 and 2 double-knockout podocytes showed dispersed acetylated alpha-tubulin and rare protrusions. In vitro, dynamin 1 polymerized around microtubules and cross-linked them into bundles, and increased their resistance to the disassembly-inducing reagents Ca(2+)and podophyllotoxin. In addition, overexpression and depletion of dynamin 1 in MPCs increased and decreased the nocodazole resistance of microtubules, respectively. These results suggest that dynamin 1 supports the microtubule bundle formation and participates in the stabilization of microtubules.
Keywords
dynamin
microtubules
podocyte
primary process
Published Date
2020-10-17
Publication Title
FASEB Journal
Volume
volume34
Issue
issue12
Publisher
Wiley
Start Page
16449
End Page
16463
ISSN
0892-6638
NCID
AA1066874X
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© 2020 The Authors.
File Version
publisher
PubMed ID
NAID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1096/fj.202001240RR
License
https://creativecommons.org/licenses/by-nc/4.0/
Funder Name
Japan Society for the Promotion of Science
助成番号
JP19H03225
JP17K08808
JP19K07084