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Title Alternative
The origin of liver epithelial-like cells derived from the normal rat
Author
Matsuura, Kazuhiko
Abstract
To investigate the origin of liver epithelial-like cells derived from the normal rat, we examined ten liver epithelial-like cell clones. These cultured cells were isolated from clonogenically growing cells in the primary culture. Morphological, cytochemical and biochemical characteristics of these ten of clones showed the followings: (1) These cells consisted of dense, diffuse and intermediate types. (2) Analyses of serum-free conditioned culture media, using SDS-PAGE, demonstrated that the dense type had a certain profile in the group of secretory proteins, whereas the diffuse and intermediate types had varied profiles although the latter were somewhat similar in profile to the dense type. (3) The secretory protein groups were different from mesenchymal cell lines in their profiles. (4) Cytokeratin was present in all the ten of clones. (5) Cytokeratin was also present in the cholangiolar epithelia and cells localized in peripheral areas of normal hepatic lobules. (6) The function, as seen in mature-type hepatocytes and cholangiolar epithelia, was almost absent. (7) These ten clones were different from sinusoidal endothelia according to their morphologies and growth ability in the primary culture. In conclusion, the liver epithelial-like cell clones derived from normal rat can belong to a “stem-cell family” of liver tissue. These “stem cells” may be localized next to the hepatocytes and cholangiolar epithelia in vivo.
Keywords
ラット
肝細胞培養
上皮様細胞
cytokeratin
stem cell
Published Date
1989-12
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume101
Issue
issue11-12
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
1037
End Page
1048
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/101/11-12/101_11-12_1037/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma