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ID 19498
Eprint ID
19498
FullText URL
68_485.pdf 668 KB
Title Alternative
Studies on the Insulin Complementfixation No. 1. Method of enlarging 24 hours of complementfixing at low temperature on Insulin Complemntfixation
Author
Morishita, Takashi
Abstract
Complement-fixing antibodies were found from rabbit sensitized by simple injection of Insulin (fish Insulin & animal Insulin), without hitherto-used horse serum etc. 1) By the cross reaction between fish Insulin and animal Insulin, Insulin complement-fixing antibodies were found after one hour of fixing antigen, antibodies and complement at 37℃. and prolonged 24 hours at 3℃; which were never found by Kolmer method. Insulin, as its molecular weight is small, takes a long time to react Insulin complement-fixing just as the case of Virus. 2) Insulin sensitized serum is positive, when complement-fixing reaction is made using extracts of fish and beef as antigen. This shows that the serum contains foreign matters besides Insulin antigen. 3) The same results were obtained by using crystal Insulin as well as commercial Insulin. 4) Insulin complement fixing antibodies last 3 or 6 months, and strongest reactions are found 7 days after the sensitization. 5) Complement-fixing antibodies were found by a cross reaction rather than by serum of patients treated by Insulin.
Published Date
1956-06-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume68
Issue
issue6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
485
End Page
494
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/68/6/68_6_485/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma