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ID 18564
Eprint ID
18564
FullText URL
75_523.pdf 5.62 MB
Title Alternative
Studies on Fluorescence Staining of Cancer Cells and Cancer Chemotherapy with Concurrent Use of Surfactants 1. Staining Mechanism of Cancer Cells By Fluorescent NTS Dye
Author
Morishita, Kazuo
Abstract
For the purpose of elucibating the staining mechanism of fluorescent NTS dye that deeply stains cancer cells, some model experiments were conducted using fluorescent NTS dye mixed with a variety of proteins to pursue the manner of staining by means of stripping method at varying pHs as well as by paper electrophoresis. In addition, staining of cells was performed with the use of Ehrlich ascites tumor cells, MH-134 ascites liver cancer cells, normal rabbit liver cells, normal rabbit leucocytes, lymph nodes and peripheral blood of gastric cancer bearing patients, and human pus, and the following results were obtained. 1. Static electrical binding seems to play a principal role in the combination of fluorescent NTS dye with various proteins. 2. Cancer cells are stained deeply with the fluorescent NTS dye at higher pH range as compared with normal cells. 3. From these findings it has been clarified that, although fluorescent NTS dye is a suitable material for staining cancer cells, as young cells with abundant basophilic proteins possess specificity, fluorescent NTS dye is not necessarily so satisfactory a material with respect to its specificity to cancer cells.
Published Date
1963-06-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume75
Issue
issue4-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
523
End Page
532
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/75/4-6/75_4-6_523/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma