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ID 16942
Eprint ID
16942
FullText URL
94_995.pdf 1.85 MB
Title Alternative
Selective silver stain of lysine-rich basic proteins separated in polyacrylamide gels.
Author
Nagai, Hiroshi
Abstract
A simple silver stain for selective visualization of lysine-rich proteins in polyacrylamide gels was developed. The specificity of the stain was confirmed with a variety of protein mixtures. The staining procedure requires a relatively short time (2.5~3 hours) and has a sensitivity comparable to that of the conventional Coomassie blue stain (about 0.1μg per band for lysine-rich histones). Differences between the new staining procedure and other "highly sensitive non-specific silver stains" are discussed.
Keywords
銀染色
塩基性蛋白質
ポリアクリルアミド・ゲル電気泳動
Published Date
1982-12-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume94
Issue
issue11-12
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
995
End Page
1002
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/94/11-12/94_11-12_995/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma