ID | 16942 |
Eprint ID | 16942
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FullText URL | |
Title Alternative | Selective silver stain of lysine-rich basic proteins separated in polyacrylamide gels.
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Author |
Nagai, Hiroshi
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Abstract | A simple silver stain for selective visualization of lysine-rich proteins in polyacrylamide gels was developed. The specificity of the stain was confirmed with a variety of protein mixtures. The staining procedure requires a relatively short time (2.5~3 hours) and has a sensitivity comparable to that of the conventional Coomassie blue stain (about 0.1μg per band for lysine-rich histones). Differences between the new staining procedure and other "highly sensitive non-specific silver stains" are discussed.
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Keywords | 銀染色
塩基性蛋白質
ポリアクリルアミド・ゲル電気泳動
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Published Date | 1982-12-30
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Publication Title |
岡山医学会雑誌
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Publication Title Alternative | Journal of Okayama Medical Association
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Volume | volume94
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Issue | issue11-12
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Publisher | 岡山医学会
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Publisher Alternative | Okayama Medical Association
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Start Page | 995
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End Page | 1002
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ISSN | 0030-1558
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NCID | AN00032489
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Content Type |
Journal Article
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Official Url | https://www.jstage.jst.go.jp/article/joma1947/94/11-12/94_11-12_995/_article/-char/ja/
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Related Url | http://www.okayama-u.ac.jp/user/oma/index.html
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language |
Japanese
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Copyright Holders | 岡山医学会
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File Version | publisher
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Refereed |
True
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Eprints Journal Name | joma
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