ID | 57388 |
フルテキストURL | |
著者 |
Yamanashi, Taro
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Maki, Misayo
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kojima, Keiichi
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Shibukawa, Atsushi
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Tsukamoto, Takashi
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Chowdhury, Srikanta
Yamanaka, Akihiro
Takagi, Shin
Sudo, Yuki
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
ORCID
Kaken ID
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抄録 | Ion pumps and channels are responsible for a wide variety of biological functions. Ion pumps transport only one ion during each stimulus-dependent reaction cycle, whereas ion channels conduct a large number of ions during each cycle. Ion pumping rhodopsins such as archaerhodopsin-3 (Arch) are often utilized as light-dependent neural silencers in animals, but they require a high-density light illumination of around 1 mW/mm2. Recently, anion channelrhodopsins -1 and -2 (GtACR1 and GtACR2) were discovered as light-gated anion channels from the cryptophyte algae Guillardia theta. GtACRs are therefore expected to silence neural activity much more efficiently than Arch. In this study, we successfully expressed GtACRs in neurons of the nematode Caenorhabditis elegans (C. elegans) and quantitatively evaluated how potently GtACRs can silence neurons in freely moving C. elegans. The results showed that the light intensity required for GtACRs to cause locomotion paralysis was around 1 µW/mm2, which is three orders of magnitude smaller than the light intensity required for Arch. As attractive features, GtACRs are less harmfulness to worms and allow stable neural silencing effects under long-term illumination. Our findings thus demonstrate that GtACRs possess a hypersensitive neural silencing activity in C. elegans and are promising tools for long-term neural silencing.
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発行日 | 2019-05-27
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出版物タイトル |
Scientific reports
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巻 | 9巻
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号 | 1号
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出版者 | Nature Publishing Group
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開始ページ | 7863
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ISSN | 20452322
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
関連URL | isVersionOf https://doi.org/10.1038/s41598-019-44308-x
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ライセンス | http://creativecommons.org/licenses/by/4.0/
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