フルテキストURL 17P-SO3-Sia-SR.pdf
著者 Vavricka, Christopher J.| Muto, Chiaki| Hasunuma, Tomohisa| Kimura, Yoshinobu| Araki, Michihiro| Wu, Yan| Gao, George F.| Ohrui, Hiroshi| Izumi, Minoru| Kiyota, Hiromasa|
抄録 The design, synthesis and application of N-acetylneuraminic acid-derived compounds bearing anomeric sulfo functional groups are described. These novel compounds, which we refer to as sulfo-sialic acid analogues, include 2-decarboxy-2-deoxy-2-sulfo-N-acetylneuraminic acid and its 4-deoxy-3,4-dehydrogenated pseudoglycal. While 2-decarboxy-2-deoxy-2-sulfo-N-acetylneuraminic acid contains no further modifications of the 2-deoxy-pyranose ring, it is still a more potent inhibitor of avian-origin H5N1 neuraminidase (NA) and drug-resistant His275Tyr NA as compared to the oxocarbenium ion transition state analogue 2,3-dehydro-2-deoxy-N-acetylneuraminic acid. The sulfo-sialic acid analogues described in this report are also more potent inhibitors of influenza NA (up to 40-fold) and bacterial NA (up to 8.5-fold) relative to the corresponding anomeric phosphonic acids. These results confirm that this novel anomeric sulfo modification offers great potential to improve the potency of next-generation NA inhibitors including covalent inhibitors.
キーワード Antiviral agents Drug discovery and development Glycosides
発行日 2017-08
出版物タイトル Scientific Reports
7巻
1号
出版者 Nature Publishing Group
開始ページ 8239
ISSN 2045-2322
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン publisher
PubMed ID 28811524
DOI 10.1038/s41598-017-07836-y
Web of Sience KeyUT 000407570000116
関連URL https://doi.org/10.1038/s41598-017-07836-y
フルテキストURL SciRep_7_1_7826.pdf
著者 Takatani, Shogo| Ozawa, Shinichiro| Yagi, Noriyoshi| Hotta, Takashi| Hashimoto, Takashi| Takahashi, Yuichiro| Takahashi, Taku| Motose, Hiroyasu|
抄録  Plant cortical microtubules align perpendicular to the growth axis to determine the direction of cell growth. However, it remains unclear how plant cells form well-organized cortical microtubule arrays in the absence of a centrosome. In this study, we investigated the functions of Arabidopsis NIMA-related kinase 6 (NEK6), which regulates microtubule organization during anisotropic cell expansion. Quantitative analysis of hypocotyl cell growth in the nek6-1 mutant demonstrated that NEK6 suppresses ectopic outgrowth and promotes cell elongation in different regions of the hypocotyl. Loss of NEK6 function led to excessive microtubule waving and distortion, implying that NEK6 suppresses the aberrant cortical microtubules. Live cell imaging showed that NEK6 localizes to the microtubule lattice and to the shrinking plus and minus ends of microtubules. In agreement with this observation, the induced overexpression of NEK6 reduced and disorganized cortical microtubules and suppressed cell elongation. Furthermore, we identified five phosphorylation sites in β-tubulin that serve as substrates for NEK6 in vitro. Alanine substitution of the phosphorylation site Thr166 promoted incorporation of mutant β-tubulin into microtubules. Taken together, these results suggest that NEK6 promotes directional cell growth through phosphorylation of β-tubulin and the resulting destabilization of cortical microtubules.
キーワード Cell growth Microtubules Plant cytoskeleton
備考 Published in Scientific Reports. 7, 7826(2017)
発行日 2017-08-10
出版物タイトル Scientific Reports
7巻
出版者 Nature Publishing Group
開始ページ 7826
ISSN 2045-23
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン publisher
PubMed ID 28798328
DOI 10.1038/s41598-017-08453-5
Web of Sience KeyUT 000407400500058
関連URL https://doi.org/10.1038/s41598-017-08453-5
フルテキストURL natute_539_7627_81.pdf natute_539_7627_81_methods.pdf natute_539_7627_81_fig.pdf
著者 Tsujino, Noriyoshi| Nishihara, Yu| Yamazaki, Daisuke| Seto, Yusuke| Higo, Yuji| Takahashi, Eiichi|
抄録  Seismic shear wave anisotropy is observed in Earth's uppermost lower mantle around several subducted slabs. The anisotropy caused by the deformation-induced crystallographic preferred orientation (CPO) of bridgmanite (perovskite-structured (Mg,Fe)SiO3) is the most plausible explanation for these seismic observations. However, the rheological properties of bridgmanite are largely unknown. Uniaxial deformation experiments have been carried out to determine the deformation texture of bridgmanite, but the dominant slip system (the slip direction and plane) has not been determined. Here we report the CPO pattern and dominant slip system of bridgmanite under conditions that correspond to the uppermost lower mantle (25 gigapascals and 1,873 kelvin) obtained through simple shear deformation experiments using the Kawai-type deformation-DIA apparatus. The fabrics obtained are characterized by [100] perpendicular to the shear plane and [001] parallel to the shear direction, implying that the dominant slip system of bridgmanite is [001](100). The observed seismic shear- wave anisotropies near several subducted slabs (Tonga-Kermadec, Kurile, Peru and Java) can be explained in terms of the CPO of bridgmanite as induced by mantle flow parallel to the direction of subduction.
キーワード Geophysics Geodynamics Mineralogy
発行日 2016-11
出版物タイトル Nature
539巻
7627号
出版者 Nature Publishing Group
開始ページ 81
終了ページ 84
ISSN 0028-0836
NCID AA00752384
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
PubMed ID 27750277
DOI 10.1038/nature19777
Web of Sience KeyUT 000386670100033
関連URL https://doi.org/10.1038/nature19777
フルテキストURL Nature_543_7643_131.pdf
著者 Suga, Michihiro| Akita, Fusamichi| Sugahara, Michihiro| Kubo, Minoru| Nakajima, Yoshiki| Nakane, Takanori| Yamashita, Keitaro| Umena, Yasufumi| Nakabayashi, Makoto| Yamane, Takahiro| Nakano, Takamitsu| Suzuki, Mamoru| Masuda, Tetsuya| Inoue, Shigeyuki| Kimura, Tetsunari| Nomura, Takashi| Yonekura, Shinichiro| Yu, Long-Jiang| Sakamoto, Tomohiro| Motomura, Taiki| Chen, Jing-Hua| Kato, Yuki| Noguchi, Takumi| Tono, Kensuke| Joti, Yasumasa| Kameshima, Takashi| Hatsui, Takaki| Nango, Eriko| Tanaka, Rie| Naitow, Hisashi| Matsuura, Yoshinori| Yamashita, Ayumi| Yamamoto, Masaki| Nureki, Osamu| Yabashi, Makina| Ishikawa, Tetsuya| Iwata, So| Shen, Jian-Ren|
抄録 Photosystem II (PSII) is a huge membrane-protein complex consisting of 20 different subunits with a total molecular mass of 350 kDa for a monomer. It catalyses light-driven water oxidation at its catalytic centre, the oxygen-evolving complex (OEC). The structure of PSII has been analysed at 1.9 Å resolution by synchrotron radiation X-rays, which revealed that the OEC is a Mn4CaO5 cluster organized in an asymmetric, 'distorted-chair' form. This structure was further analysed with femtosecond X-ray free electron lasers (XFEL), providing the 'radiation damage-free' structure. The mechanism of O=O bond formation, however, remains obscure owing to the lack of intermediate-state structures. Here we describe the structural changes in PSII induced by two-flash illumination at room temperature at a resolution of 2.35 Å using time-resolved serial femtosecond crystallography with an XFEL provided by the SPring-8 ångström compact free-electron laser. An isomorphous difference Fourier map between the two-flash and dark-adapted states revealed two areas of apparent changes: around the QB/non-haem iron and the Mn4CaO5 cluster. The changes around the QB/non-haem iron region reflected the electron and proton transfers induced by the two-flash illumination. In the region around the OEC, a water molecule located 3.5 Å from the Mn4CaO5 cluster disappeared from the map upon two-flash illumination. This reduced the distance between another water molecule and the oxygen atom O4, suggesting that proton transfer also occurred. Importantly, the two-flash-minus-dark isomorphous difference Fourier map showed an apparent positive peak around O5, a unique μ4-oxo-bridge located in the quasi-centre of Mn1 and Mn4 (refs 4,5). This suggests the insertion of a new oxygen atom (O6) close to O5, providing an O=O distance of 1.5 Å between these two oxygen atoms. This provides a mechanism for the O=O bond formation consistent with that proposed previously
発行日 2017-03
出版物タイトル Nature
543巻
7643号
出版者 Nature Publishing Group
開始ページ 131
終了ページ 135
ISSN 0028-0836
NCID AA00752384
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
PubMed ID 28219079
DOI 10.1038/nature21400
Web of Sience KeyUT 000395671500047
関連URL https://doi.org/10.1038/nature21400
著者 Nakatsuka, Atsuko| Matsuyama, Makoto| Yamaguchi, Satoshi| Katayama, Akihiro| Eguchi, Jun| Murakami, Kazutoshi| Teshigawara, Sanae| Ogawa, Daisuke| Wada, Nozomu| Yasunaka, Tetsuya| Ikeda, Fusao| Takaki, Akinobu| Watanabe, Eijiro| Wada, Jun|
発行日 2016-02-17
出版物タイトル Scientific Reports
6巻
資料タイプ 学術雑誌論文
著者 Tamura, Takashi| Tsunekawa, Naoki| Nemoto, Michiko| Inagaki, Kenji| Hirano, Toshiyuki| Sato, Fumitoshi|
発行日 2016-01-28
出版物タイトル Scientific reports
6巻
資料タイプ 学術雑誌論文
著者 Katayama, Akihiro| Nakatsuka, Atsuko| Eguchi, Jun| Murakami, Kazutoshi| Teshigawara, Sanae| Kanzaki, Motoko| Nunoue, Tomokazu| Hida, Kazuyuki| Wada, Nozomu| Yasunaka, Tetsuya| Ikeda, Fusao| Takaki, Akinobu| Yamamoto, Kazuhide| Kiyonari, Hiroshi| Makino, Hirofumi| Wada, Jun|
発行日 2015
出版物タイトル Scientific reports
5巻
資料タイプ 学術雑誌論文
著者 Ishikawa, Atsushi| Tanaka, Takuo|
発行日 2015
出版物タイトル Scientific reports
5巻
資料タイプ 学術雑誌論文
著者 Taketa, Hiroaki| Gulsan, Ara Sathi| Mahmoud, Farahat| Kazi, Anisur Rahman| Sakai, Takayoshi| Hirano, Yoshiaki| Kuboki, Takuo| Torii, Yasuhiro| Matsumoto, Takuya|
発行日 2015
出版物タイトル Scientific reports
5巻
資料タイプ 学術雑誌論文
著者 Katanosaka, Yuki| Iwasaki, Keiichiro| Ujihara, Yoshihiro| Takatsu, Satomi| Nishitsuji, Koki| Kanagawa, Motoi| Sudo, Atsushi| Toda, Tatsushi| Katanosaka, Kimiaki| Mohri, Satoshi| Naruse, Keiji|
発行日 2014-05-29
出版物タイトル Nature Communications
5巻
資料タイプ 学術雑誌論文
著者 Kyotaro, Ohno| Yasuharu, Sato| Koh-ichi, Ohshima| Katsuyoshi, Takata| Tomoko, Miyata-Takata| Mai, Takeuchi| Yuka, Gion| Tomoyasu, Tachibana| Yorihisa, Orita| Toshihiro, Ito| Steven, H. Swerdlow| Tadashi, Yoshino|
発行日 2015
出版物タイトル Scientific Reports
5巻
資料タイプ 学術雑誌論文
著者 Ryosuke, Nakato| Yu, Ohkubo| Akari, Konishi| Mari, Shibata| Yuki, Kaneko| Takao, Iwawaki| Tomohiro, Nakamura| Stuart A., Lipton| Takashi, Uehara|
発行日 2015
出版物タイトル Scientific Reports
5巻
資料タイプ 学術雑誌論文
著者 Suga, Michihiro| 秋田 総理| Hirata, Kunio| Ueno, Go| Murakami, Hironori| Nakajima, Yoshiki| Shimizu, Tetsuya| Yamashita, Keitaro| Yamamoto, Masaki| Ago, Hideo| 沈 建仁|
発行日 2015-01-01
出版物タイトル Nature
517巻
資料タイプ 学術雑誌論文
フルテキストURL Takatani ABA SR15 srep11364.pdf
著者 Takatani, Shogo| Hirayama, Takashi| Hashimoto, Takashi| Takahashi, Taku| Motose, Hiroyasu|
抄録 Abscisic acid (ABA) regulates seed maturation, germination and various stress responses in plants. The roles of ABA in cellular growth and morphogenesis, however, remain to be explored. Here, we report that ABA induces the ectopic outgrowth of epidermal cells in Arabidopsis thaliana. Seedlings of A. thaliana germinated and grown in the presence of ABA developed ectopic protrusions in the epidermal cells of hypocotyls, petioles and cotyledons. One protrusion was formed in the middle of each epidermal cell. In the hypocotyl epidermis, two types of cell files are arranged alternately into non-stoma cell files and stoma cell files, ectopic protrusions being restricted to the non-stoma cell files. This suggests the presence of a difference in the degree of sensitivity to ABA or in the capacity of cells to form protrusions between the two cell files. The ectopic outgrowth was suppressed in ABA insensitive mutants, whereas it was enhanced in ABA hypersensitive mutants. Interestingly, ABA-induced ectopic outgrowth was also suppressed in mutants in which microtubule organization was compromised. Furthermore, cortical microtubules were disorganized and depolymerized by the ABA treatment. These results suggest that ABA signaling induces ectopic outgrowth in epidermal cells through microtubule reorganization.
キーワード Cell growth Plant cytoskeleton
備考 Scientific Reports 5, Article number: 11364 (2015), © 2015 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. This work is licensed under a Creative Commons Attribution 4.0 International License.
発行日 2015-06-12
出版物タイトル Scientific Reports
5巻
出版者 Nature Publishing Group
開始ページ 11364
ISSN 2045-2322
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 © 2015 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.
論文のバージョン publisher
PubMed ID 26068445
DOI 10.1038/srep11364
Web of Sience KeyUT 000356152700001
著者 Miyaji, Takaaki| Kuromori, Takashi| Takeuchi, Yu| Yamaji, Naoki| Yokosho, Kengo| Shimazawa, Atsushi| Sugimoto, Eriko| Omote, Hiroshi| Ma, Jian Feng| Shinozaki, Kazuo| Moriyama, Yoshinori|
発行日 2015-01-05
出版物タイトル Nature Communications
6巻
資料タイプ 学術雑誌論文
著者 Fujii, K| Kurozumi, K| Ichikawa, T| Onishi, M| Shimazu, Y| Ishida, J| Chiocca, EA| Kaur, B| Date, I|
発行日 2013-08
出版物タイトル Cancer Gene Therapy
20巻
8号
資料タイプ 学術雑誌論文
著者 Kosaka, H| Ichikawa, T| Kurozumi, K| Kambara, H| Inoue, S| Maruo, T| Nakamura, K| Hamada, H| Date, I|
発行日 2012-08
出版物タイトル Cancer Gene Therapy
19巻
8号
資料タイプ 学術雑誌論文
著者 The International Barley Genome Sequencing Consortium|
発行日 2012-11-29
出版物タイトル Nature
491巻
7426号
資料タイプ 学術雑誌論文
著者 Huang, P| Kaku, H| Chen, J| Kashiwakura, Y| Saika, T| Nasu, Y| Urata, Y| Fujiwara, T| Watanabe, M| Kumon, H|
発行日 2010-07
出版物タイトル Cancer Gene Therapy
17巻
7号
資料タイプ 学術雑誌論文
著者 Umena, Yasufumi| Kawakami, Keisuke| Shen, Jian-Ren| Kamiya, Nobuo|
発行日 2011-05-05
出版物タイトル Nature
473巻
7345号
資料タイプ 学術雑誌論文