ID | 48570 |
フルテキストURL | |
著者 |
Nagai, Yusuke
岡山大学
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抄録 | The aim of this present study was to provide a scaffold as a tool for the investigation of the effect of mechanical stimulation on three-dimensionally cultured cells. For this purpose, we developed an artificial self-assembling peptide (SPG-178) hydrogel scaffold. The structural properties of the SPG-178 peptide were confirmed by attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and transmission electron microscopy (TEM). The mechanical properties of the SPG-178 hydrogel were studied using rheology measurements. The SPG-178 peptide was able to form a stable, transparent hydrogel in a neutral pH environment In the SPG-178 hydrogel, mouse skeletal muscle cells proliferated successfully (increased by 12.4 +/- 1.5 times during 8 days of incubation; mean +/- SEM). When the scaffold was statically stretched, a rapid phosphorylation of ERK was observed (increased by 2.8 +/- 0.2 times; mean +/- SEM). These results demonstrated that the developed self-assembling peptide gel is non-cytotoxic and is a suitable tool for the investigation of the effect of mechanical stimulation on three-dimensional cell culture.
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キーワード | Cell proliferation
Self-assembly
Hydrogel
Scaffold
Mechanical strain
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発行日 | 2012-02
|
出版物タイトル |
Biomaterials
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巻 | 33巻
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号 | 4号
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出版者 | Elsevier Ltd.
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開始ページ | 1044
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終了ページ | 1051
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ISSN | 0142-9612
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NCID | AA11522637
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資料タイプ |
学術雑誌論文
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オフィシャル URL | http://www.sciencedirect.com/science/article/pii/S0142961211012531
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関連URL | http://ousar.lib.okayama-u.ac.jp/metadata/48434
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言語 |
英語
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著作権者 | © 2011 Elsevier Ltd. All rights reserved.
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論文のバージョン | author
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査読 |
有り
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DOI | |
Web of Science KeyUT |