ID | 48570 |
FullText URL | |
Author |
Nagai, Yusuke
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Abstract | The aim of this present study was to provide a scaffold as a tool for the investigation of the effect of mechanical stimulation on three-dimensionally cultured cells. For this purpose, we developed an artificial self-assembling peptide (SPG-178) hydrogel scaffold. The structural properties of the SPG-178 peptide were confirmed by attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and transmission electron microscopy (TEM). The mechanical properties of the SPG-178 hydrogel were studied using rheology measurements. The SPG-178 peptide was able to form a stable, transparent hydrogel in a neutral pH environment In the SPG-178 hydrogel, mouse skeletal muscle cells proliferated successfully (increased by 12.4 +/- 1.5 times during 8 days of incubation; mean +/- SEM). When the scaffold was statically stretched, a rapid phosphorylation of ERK was observed (increased by 2.8 +/- 0.2 times; mean +/- SEM). These results demonstrated that the developed self-assembling peptide gel is non-cytotoxic and is a suitable tool for the investigation of the effect of mechanical stimulation on three-dimensional cell culture.
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Keywords | Cell proliferation
Self-assembly
Hydrogel
Scaffold
Mechanical strain
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Published Date | 2012-02
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Publication Title |
Biomaterials
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Volume | volume33
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Issue | issue4
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Publisher | Elsevier Ltd.
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Start Page | 1044
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End Page | 1051
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ISSN | 0142-9612
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NCID | AA11522637
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Content Type |
Journal Article
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Official Url | http://www.sciencedirect.com/science/article/pii/S0142961211012531
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Related Url | http://ousar.lib.okayama-u.ac.jp/metadata/48434
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language |
English
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Copyright Holders | © 2011 Elsevier Ltd. All rights reserved.
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File Version | author
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Refereed |
True
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DOI | |
Web of Science KeyUT |