ID | 53953 |
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著者 |
Tamura, Takashi
Graduate School of Environmental and Life Science, Okayama University
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publons
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Tsunekawa, Naoki
Institute of Industrial Science, the University of Tokyo
Nemoto, Michiko
Graduate School of Environmental and Life Science, Okayama University
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Inagaki, Kenji
Graduate School of Environmental and Life Science, Okayama University
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Hirano, Toshiyuki
Institute of Industrial Science, the University of Tokyo
Sato, Fumitoshi
Institute of Industrial Science, the University of Tokyo
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抄録 | Oxygen tolerance of selenium-containing [NiFeSe] hydrogenases (Hases) is attributable to the high reducing power of the selenocysteine residue, which sustains the bimetallic Ni–Fe catalytic center in the large subunit. Genes encoding [NiFeSe] Hases are inherited by few sulphate-reducing δ-proteobacteria globally distributed under various anoxic conditions. Ancestral sequences of [NiFeSe] Hases were elucidated and their three-dimensional structures were recreated in silico using homology modelling and molecular dynamic simulation, which suggested that deep gas channels gradually developed in [NiFeSe] Hases under absolute anaerobic conditions, whereas the enzyme remained as a sealed edifice under environmental conditions of a higher oxygen exposure risk. The development of a gas cavity appears to be driven by non-synonymous mutations, which cause subtle conformational changes locally and distantly, even including highly conserved sequence regions.
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備考 | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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発行日 | 2016-01-28
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出版物タイトル |
Scientific reports
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巻 | 6巻
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出版者 | Nature Publishing Group
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開始ページ | 19742
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ISSN | 2045-2322
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資料タイプ |
学術雑誌論文
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オフィシャル URL | http://dx.doi.org/10.1038/srep19742
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言語 |
英語
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著作権者 | © 2016 Nature Publishing Group
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論文のバージョン | publisher
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査読 |
有り
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