著者 Yasuda, Yukiko| Sakai, Akiko| Ito, Sachio| Sasai, Kaori| Yamamoto, Hiromasa| Matsubara, Nagahide| Ouchida, Mamoru| Katayama, Hiroshi| Shimizu, Kenji|
発行日 2017-02
出版物タイトル Acta Medica Okayama
71巻
1号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/54826
著者 Hayashi, Keiichiro| Ueshima, Satoshi| Ouchida, Mamoru| Mashimo, Tomoji| Nishiki, Teiichi| Sendo, Toshiaki| Serikawa, Tadao| Matsui, Hideki| Ohmori, Iori|
発行日 2011-05
出版物タイトル Epilepsia
52巻
5号
資料タイプ 学術雑誌論文
著者 Yoneda, Yasushi| Ito, Sachio| Kunisada, Toshiyuki| Morimoto, Yuki| Kanzaki, Hirotaka| Yoshida, Aki| Shimizu, Kenji| Ozaki, Toshifumi| Ouchida, Mamoru|
発行日 2013-10-09
出版物タイトル PLoS ONE
8巻
10号
資料タイプ 学術雑誌論文
著者 Maruo, Tomoko| Ichikawa, Tomotsugu| Kanzaki, Hirotaka| Inoue, Satoshi| Kurozumi, Kazuhiko| Onishi, Manabu| Yoshida, Koichi| Kambara, Hirokazu| Ouchida, Mamoru| Shimizu, Kenji| Tamaru, Seiji| Chiocca, E. Antonio| Date, Isao|
発行日 2013-06
出版物タイトル Neuropathology
33巻
3号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/49042
フルテキストURL 66_6_461.pdf
著者 Koike, Kazuko| Takaki, Akinobu| Kato, Nobuyuki| Ouchida, Mamoru| Kanzaki, Hirotaka| Yasunaka, Tetsuya| Shiraha, Hidenori| Miyake, Yasuhiro| Yamamoto, Kazuhide|
抄録 Hepatitis C virus (HCV) infection induces several changes in hepatocytes, such as oxidative stress, steatosis, and hepatocarcinogenesis. Although considerable progress has been made during recent years, the mechanisms underlying these functions remain unclear. We employed proteomic techniques in HCV replicon-harboring cells to determine the effects of HCV replication on host-cell protein expression. We examined two-dimensional electrophoresis (2-DE) and mass spectrometry to compare and identify differentially expressed proteins between HCV subgenomic replicon-harboring cells and their “cured” cells. One of the identified proteins was confirmed using enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. Full-length HCV genome RNA replicating and cured cells were also assessed using ELISA. Replicon-harboring cells showed higher expression of retinal dehydrogenase 1 (RALDH-1), which converts retinol to retinoic acid, and the cured cells showed higher expression of retinol-binding protein (RBP), which transports retinol from the liver to target tissues. The alteration in RBP expression was also confirmed by ELISA and Western blot analysis. We conclude that protein expression profiling demonstrated that HCV replicon eradication affected retinol-related protein expression.
キーワード hepatitis C virus retinol-binding protein
Amo Type Original Article
発行日 2012-12
出版物タイトル Acta Medica Okayama
66巻
6号
出版者 Okayama University Medical School
開始ページ 461
終了ページ 468
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2012 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 23254580
Web of Sience KeyUT 000312966100005
JaLCDOI 10.18926/AMO/48569
フルテキストURL 66_3_285.pdf
著者 Mizobuchi, Satoshi| Matsuoka, Yoshikazu| Obata, Norihiko| Kaku, Ryuji| Itano, Yoshitaro| Tomotsuka, Naoto| Taniguchi, Arata| Nishie, Hiroyuki| Kanzaki, Hirotaka| Ouchida, Mamoru| Morita, Kiyoshi|
抄録 Perioperative beta-blocker administration has recently been recommended for patients undergoing cardiac or other surgery due to the beneficial cardiovascular effects of these agents. In addition, some studies have reported that perioperatively administered beta-blockers also have analgesic effects. In this study, to investigate the antinociceptive effects and the analgesic profile of landiolol, we examined the effects of intrathecal landiolol administration on nociceptive pain behavior and c-fos mRNA expression (a neural marker of pain) in the spinal cord using a rat formalin model. We found that pain-related behavior was inhibited by intrathecal landiolol administration. Moreover, the increase in c-fos mRNA expression on the formalin-injected side was less pronounced in rats administered landiolol than in saline administered controls. Thus, intrathecal administration of landiolol exhibited antinociceptive effects. Further investigation of the antinociceptive mechanism of landiolol is required.
キーワード beta-blocker landiolol formalin pain behavior c-fos
Amo Type Original Article
発行日 2012-06
出版物タイトル Acta Medica Okayama
66巻
3号
出版者 Okayama University Medical School
開始ページ 285
終了ページ 289
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2012 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 22729110
Web of Sience KeyUT 000305669700013
JaLCDOI 10.18926/AMO/48262
フルテキストURL 66_2_119.pdf.pdf
著者 Oka, Hiroaki| Ouchida, Mamoru| Kondo, Takuya| Morita, Fumio| Shimizu, Kenji|
抄録 Human lymphoblastoid TK6 and WTK-1 cells are widely used to detect mutagens in vitro. TK6 cells have wild-type TP53 alleles, while WTK-1 cells have one allele of mutated TP53. Both cells were treated with 5-fluorouracil (5-FU), and gene mutation assay and micronucleus assay were performed to clarify the differential response related to the TP53 gene status. The effects of 5-FU on gene expression were assessed by microarray and quantitative RT-PCR analyses. In WTK-1 cells, 5-FU increased the frequency of cells with micronucleus and mutation. In TK6 cells, frequency of cells with micronucleus was increased but the mutation frequency was not. The cytotoxicity induced by 5-FU was more prominent in TK6 cells than in WTK-1 cells. Analysis of gene expression showed that the genes involved in the TP53 pathway were up-regulated in TK6 cells but not in WTK-1 cells. The differential responses to 5-FU between these cell lines appeared to be due to the difference in the TP53 gene status, thus providing a molecular basis for the bioassays using these cell lines in the toxicology field. Our results indicate that the clinical efficacy of 5-FU chemotherapy may depend on the TP53 genotype.
キーワード 5-fluorouracil TP53 Tk mutation assays microarray analysis
Amo Type Original Article
発行日 2012-04
出版物タイトル Acta Medica Okayama
66巻
2号
出版者 Okayama University Medical School
開始ページ 119
終了ページ 129
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2012 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 22525470
Web of Sience KeyUT 000303175300005
著者 Yano, Masaaki| Ouchida, Mamoru| Shigematsu, Hisayuki| Tanaka, Noriyoshi| Ichimura, Koichi| Kobayashi, Kazuyasu| Inaki, Yasuhiko| Toyooka, Shinichi| Tsukuda, Kazunori| Shimizu, Nobuyoshi| Shimizu, Kenji|
発行日 2004-10-20
出版物タイトル International Journal of Cancer
112巻
1号
資料タイプ 学術雑誌論文
著者 Ito, Tatsuo| Ouchida, Mamoru| Morimoto, Yuki| Yoshida, Aki| Jitsumori, Yoshimi| Ozaki, Toshifumi| Sonobe, Hiroshi| Inoue, Hajime| Shimizu, Kenji|
発行日 2005-06-28
出版物タイトル Cancer Letters
224巻
2号
資料タイプ 学術雑誌論文
著者 大守 伊織| 大内田 守| 御牧 信義| 西木 禎一| 富澤 一仁| 松井 秀樹|
発行日 2009-12-01
出版物タイトル 岡山医学会雑誌
121巻
3号
資料タイプ 学術雑誌論文
著者 末久 弘| 豊岡 伸一| 堀田 勝幸| 内田 亜希子| 宗 淳一| 藤原 義朗| 松尾 恵太郎| 大内田 守| 高田 穣| 木浦 勝行| 伊達 洋至|
発行日 2008-12-01
出版物タイトル 岡山医学会雑誌
120巻
3号
資料タイプ 学術雑誌論文
著者 石井 龍宏| 村上 純| 能登原 憲司| 笹本 博美| 神原 健| 白川 靖博| 猶本 良夫| 大内田 守| 清水 憲二| Jeremy, R. Jass| 松原 長秀| 田中 紀章|
発行日 2007-09-03
出版物タイトル 岡山医学会雑誌
119巻
2号
資料タイプ 学術雑誌論文