ID | 60847 |
フルテキストURL | |
著者 |
La, The Mon
Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Tachibana, Hiromi
Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Li, Shun-Ai
Center for Innovative Clinical Medicine, Okayama University Hospital
Abe, Tadashi
Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Seiriki, Sayaka
Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Nagaoka, Hikaru
Division of Malaria Research, Proteo-Science Center, Ehime University
Takashima, Eizo
Division of Malaria Research, Proteo-Science Center, Ehime University
Takeda, Tetsuya
Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Ogawa, Daisuke
Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kaken ID
Makino, Shin-Ichi
Department of Nephrology, Graduate School of Medicine, Chiba University
Asanuma, Katsuhiko
Department of Nephrology, Graduate School of Medicine, Chiba University
Watanabe, Masami
Center for Innovative Clinical Medicine, Okayama University Hospital
Kaken ID
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Tian, Xuefei
Department of Internal Medicine, Section of Nephrology, Yale University School of Medicine
Ishibe, Shuta
Department of Internal Medicine, Section of Nephrology, Yale University School of Medicine
Sakane, Ayuko
Department of Biochemistry, Tokushima University Graduate School of Medical Sciences
Sasaki, Takuya
Department of Biochemistry, Tokushima University Graduate School of Medical Sciences
Wada, Jun
Department of Nephrology, Rheumatology, Endocrinology and Metabolism, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
ORCID
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Takei, Kohji
Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kaken ID
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Yamada, Hiroshi
Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kaken ID
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抄録 | Dynamin 1 is a neuronal endocytic protein that participates in vesicle formation by scission of invaginated membranes. Dynamin 1 is also expressed in the kidney; however, its physiological significance to this organ remains unknown. Here, we show that dynamin 1 is crucial for microtubule organization and stabilization in glomerular podocytes. By immunofluorescence and immunoelectron microscopy, dynamin 1 was concentrated at microtubules at primary processes in rat podocytes. By immunofluorescence of differentiated mouse podocytes (MPCs), dynamin 1 was often colocalized with microtubule bundles, which radially arranged toward periphery of expanded podocyte. In dynamin 1-depleted MPCs by RNAi, alpha-tubulin showed a dispersed linear filament-like localization, and microtubule bundles were rarely observed. Furthermore, dynamin 1 depletion resulted in the formation of discontinuous, short acetylated alpha-tubulin fragments, and the decrease of microtubule-rich protrusions. Dynamins 1 and 2 double-knockout podocytes showed dispersed acetylated alpha-tubulin and rare protrusions. In vitro, dynamin 1 polymerized around microtubules and cross-linked them into bundles, and increased their resistance to the disassembly-inducing reagents Ca(2+)and podophyllotoxin. In addition, overexpression and depletion of dynamin 1 in MPCs increased and decreased the nocodazole resistance of microtubules, respectively. These results suggest that dynamin 1 supports the microtubule bundle formation and participates in the stabilization of microtubules.
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キーワード | dynamin
microtubules
podocyte
primary process
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発行日 | 2020-10-17
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出版物タイトル |
FASEB Journal
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巻 | 34巻
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号 | 12号
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出版者 | Wiley
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開始ページ | 16449
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終了ページ | 16463
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ISSN | 0892-6638
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NCID | AA1066874X
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2020 The Authors.
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論文のバージョン | publisher
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PubMed ID | |
NAID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.1096/fj.202001240RR
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ライセンス | https://creativecommons.org/licenses/by-nc/4.0/
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助成機関名 |
日本学術振興会
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助成番号 | JP19H03225
JP17K08808
JP19K07084
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