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ID 32814
JaLCDOI
フルテキストURL
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著者
Okamoto, Akira Okayama University
Yamamura, Masahiro Okayama University
Iwahashi, Mitsuhiro Okayama University
Aita, Tetsushi Okayama University
Ueno, Akiko Okayama University
Kawashima, Masanori Okayama University
Yamana, Jiro Okayama University
Kagawa, Hidetoshi Okayama University
Makino, Hirofumi Okayama University
抄録

High levels of soluble CD30 (sCD30) were detected in the serum and synovial fluid of patients with rheumatoid arthritis (RA), indicating the involvement of CD30+ T cells in the pathogenesis. We investigated the induction of CD30 and its functions in CD4+T cells from patients with established RA (disease duration >_2 years). CD4+ T cells from both the peripheral blood (PB) and synovial tissue (ST) of RA patients expressed surface CD30 when stimulated with anti-CD3 antibody (Ab) and anti-CD28 Ab, but their CD30 induction was slower and weaker compared with PB CD4+ T cells of healthy controls (HC). Immunohistochemical analysis showed that only a small proportion of lymphocytes expressed CD30 in the ST (-1%). RA PB CD4+ T cells, after recovery from 6-day stimulation with anti-CD3 Ab and anti-CD28 Ab, showed in intracellular cytokine staining that CD30+ T cells could produce more interleukin-4 (IL-4) but less interferon-gamma. In the culture of RA PB CD4+ T Cells with anti-CD3 Ab and anti-CD28 Ab, blocking anti-CD30 Ab similarly inhibited the cell proliferation and activation of nuclear factor-kappaB on day 4 in RA and HC, but inhibited the apoptotic cell death on day 6 only in RA. These results indicate that despite high-level expression of sCD30, the anti-inflammatory activity of IL-4-producing CD30+ CD4+ T cells may be limited in the ST due to a poor induction of surface CD30 and a susceptibility to CD30-mediated cell death.

キーワード
apoptosis
CD4 Tcells
CD30
interleukin-4(IL=4)
rheumatoid arthritis(RA)
Amo Type
Article
発行日
2003-12
出版物タイトル
Acta Medica Okayama
57巻
6号
出版者
Okayama University Medical School
開始ページ
267
終了ページ
277
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Science KeyUT