ID | 32814 |
JaLCDOI | |
フルテキストURL | |
著者 |
Okamoto, Akira
Okayama University
Yamamura, Masahiro
Okayama University
Iwahashi, Mitsuhiro
Okayama University
Aita, Tetsushi
Okayama University
Ueno, Akiko
Okayama University
Kawashima, Masanori
Okayama University
Yamana, Jiro
Okayama University
Kagawa, Hidetoshi
Okayama University
Makino, Hirofumi
Okayama University
|
抄録 | High levels of soluble CD30 (sCD30) were detected in the serum and synovial fluid of patients with rheumatoid arthritis (RA), indicating the involvement of CD30+ T cells in the pathogenesis. We investigated the induction of CD30 and its functions in CD4+T cells from patients with established RA (disease duration >_2 years). CD4+ T cells from both the peripheral blood (PB) and synovial tissue (ST) of RA patients expressed surface CD30 when stimulated with anti-CD3 antibody (Ab) and anti-CD28 Ab, but their CD30 induction was slower and weaker compared with PB CD4+ T cells of healthy controls (HC). Immunohistochemical analysis showed that only a small proportion of lymphocytes expressed CD30 in the ST (-1%). RA PB CD4+ T cells, after recovery from 6-day stimulation with anti-CD3 Ab and anti-CD28 Ab, showed in intracellular cytokine staining that CD30+ T cells could produce more interleukin-4 (IL-4) but less interferon-gamma. In the culture of RA PB CD4+ T Cells with anti-CD3 Ab and anti-CD28 Ab, blocking anti-CD30 Ab similarly inhibited the cell proliferation and activation of nuclear factor-kappaB on day 4 in RA and HC, but inhibited the apoptotic cell death on day 6 only in RA. These results indicate that despite high-level expression of sCD30, the anti-inflammatory activity of IL-4-producing CD30+ CD4+ T cells may be limited in the ST due to a poor induction of surface CD30 and a susceptibility to CD30-mediated cell death. |
キーワード | apoptosis
CD4 Tcells
CD30
interleukin-4(IL=4)
rheumatoid arthritis(RA)
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 2003-12
|
巻 | 57巻
|
号 | 6号
|
出版者 | Okayama University Medical School
|
開始ページ | 267
|
終了ページ | 277
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
|
PubMed ID | |
Web of Science KeyUT |