| ID | 52795 |
| フルテキストURL | |
| 著者 |
Hirai, Misato
Okayama Univ, Grad Sch Med Dent & Pharm, Dept Otolaryngol Head & Neck Surg
Maeda, Yukihide
Okayama Univ, Grad Sch Med Dent & Pharm, Dept Otolaryngol Head & Neck Surg
Kaken ID
publons
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Fukushima, Kunihiro
Okayama Univ, Grad Sch Med Dent & Pharm, Dept Otolaryngol Head & Neck Surg
Sugaya, Akiko
Okayama Univ, Grad Sch Med Dent & Pharm, Dept Otolaryngol Head & Neck Surg
Kataoka, Yuko
Okayama Univ, Grad Sch Med Dent & Pharm, Dept Otolaryngol Head & Neck Surg
Kaken ID
publons
Nishizaki, Kazunori
Okayama Univ, Grad Sch Med Dent & Pharm, Dept Otolaryngol Head & Neck Surg
Kaken ID
publons
researchmap
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| 抄録 | MicroRNAs (miRNAs) play functional roles in sound transduction in cochlea. This study focuses on the validity of cochlear culture as an in vitro experimental tool, in view of miRNA expression. E15 cochleae were dissected and maintained in vitro for 48 h before extraction of miRNAs. MiRNA expression was comprehensively screened in explanted cochleae using a miRNA array that covers 380 miRNAs. A strong correlation was observed between expression levels of miRNAs in in vitro and in in vivo cochleae. Levels of 43 miRNAs were altered in vitro and these changes were reproducible over three trials. These findings indicate that in vitro miRNA profiling is a viable method for analysis of gene expression and action of chemical compounds on cochleae.
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| キーワード | embryonic cochlea
microRNA array
organ culture of cochlea
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| 発行日 | 2011-09-14
|
| 出版物タイトル |
NeuroReport
|
| 巻 | 22巻
|
| 号 | 13号
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| 開始ページ | 652
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| 終了ページ | 654
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| ISSN | 0959-4965
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| 資料タイプ |
学術雑誌論文
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| 関連URL | http://ousar.lib.okayama-u.ac.jp/metadata/52518
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| 言語 |
英語
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| 著作権者 | (C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
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| 論文のバージョン | author
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| 査読 |
有り
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| DOI | |
| Web of Science KeyUT |
