Efficacy and safety of spot heating and ultrasound irradiation on in vitro and in vivo thrombolysis models

Morihara, Ryuta; Yamashita, Toru; Osakada, Yosuke; Feng, Tian; Hu, Xinran; Fukui, Yusuke; Tadokoro, Koh; Takemoto, Mami; Abe, Koji

doi:10.1177/0271678x221079127

Permalink : http://escholarship.lib.okayama-u.ac.jp/63260

ID	63260
フルテキストURL	fulltext20220309-2.pdf 291 KB Figure20220309-2.pdf 789 KB Supplemental figure.pdf 1010 KB
著者	Morihara, Ryuta Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences ORCID Kaken ID researchmap Yamashita, Toru Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences ORCID Kaken ID researchmap Osakada, Yosuke Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences ORCID Feng, Tian Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Hu, Xinran Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Fukui, Yusuke Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan Tadokoro, Koh Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Takemoto, Mami Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences ORCID Kaken ID Abe, Koji Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Kaken ID publons researchmap
抄録	The feasibility of transcranial sonothrombolysis has been demonstrated, although little is known about the relationships between thermal or mechanical mechanisms and thrombolytic outcomes. Therefore, the present study aims to reveal the effect and safety of temperature and ultrasound through in vitro and in vivo thrombolysis models. Artificial clots in microtubes were heated in a water bath or sonicated by ultrasound irradiation, and then clots weight decrease with rising temperature and sonication time was confirmed. In the in vitro thrombotic occlusion model, based on spot heating, clot volume was reduced and clots moved to the distal side, followed by recanalization of the occlusion. In the in vivo study, the common carotid artery of rats was exposed to a spot heater or to sonication. No brain infarct or brain blood barrier disruption was shown, but endothelial junctional dysintegrity and an inflammatory response in the carotid artery were detected. The present spot heating and ultrasound irradiation models seem to be effective for disintegrating clots in vitro, but the safety of the in vivo model was not fully supported by the data. However, the data indicates that a shorter time exposure could be less invasive than a longer exposure.
備考	This is an Accepted Manuscript published by SAGE Publications.
発行日	2022-2-8
出版物タイトル	Journal of Cerebral Blood Flow & Metabolism
出版者	SAGE Publications
ISSN	0271-678X
NCID	AA10458430
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	Copyright © 2022 by International Society for Cerebral Blood Flow and Metabolism
論文のバージョン	author
DOI	10.1177/0271678x221079127
関連URL	isVersionOf https://doi.org/10.1177/0271678x221079127