JaLCDOI | 10.18926/AMO/31681 |
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フルテキストURL | fulltext.pdf |
著者 | Iguchi, Hiroki| Watanabe, Masami| Kamitani, Akihiro| Nagai, Atsushi| Hosoya, Osamu| Tsutsui, Kimiko| Kumon, Hiromi| |
抄録 | Dynamin is a protein essential to endocytosis. Dynamin 2, a dynamin isoform, is expressed most intensely in testicular tissue; however, precise localization has never been studied. Therefore, we investigated the expression of dynamin 2 in rat testicular tissue using immunohistochemical methods, and discuss here the physiological function of this protein. Testicular tissues were obtained from Wistar rats at 10, 21 and 63 days of age. Immunohistochemistrical examination and Western blot analysis were conducted using dynamin 2 specific antibody. Western blot analysis showed that expression in 21- and 63-day-old rats was more intense than that in 10-day-old rats. Dynamin 2 expression was observed using immunohistochemical method in the seminiferous tubules of all rats. In the 63-day-old rats, the expression was intense, especially in spermatids in the earlier maturation stages and in spermatocytes, and was observed in Sertoli cells. However, in spermatids, the expression gradually declined as spermatids matured to spermatozoa. In the 21-day-old rats, the expression was evident in spermatocytes and Sertoli cells, but that in the 10-day-old rats was weak. Intense expression of dynamin 2 during spermatogenesis suggests that this protein plays an important role in this process. |
キーワード | dynamin 2 endocytosis spermatogenesis |
Amo Type | Article |
出版物タイトル | Acta Medica Okayama |
発行日 | 2002-08 |
巻 | 56巻 |
号 | 4号 |
出版者 | Okayama University Medical School |
開始ページ | 205 |
終了ページ | 209 |
ISSN | 0386-300X |
NCID | AA00508441 |
資料タイプ | 学術雑誌論文 |
言語 | 英語 |
論文のバージョン | publisher |
査読 | 有り |
PubMed ID | 12199526 |
Web of Science KeyUT | 000177382600006 |
JaLCDOI | 10.18926/AMO/31512 |
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フルテキストURL | fulltext.pdf |
著者 | Tsutsui, Ken| Tsutsui, Kimiko| Aoyama, Koji| Oda, Takuzo| |
抄録 | The extent of homology between two protein fractions was compared by simple electrophoretic analysis. Nuclear proteins of several rodent cells of different origins were fractionated into acid-soluble and acid-insoluble fractions. The two protein fractions were subjected to polyacrylamide gel electrophoresis in separate gel systems, and protein bands with identical mobilities were sought either in all possible combinational pairs of cell types or in all cell types. The paired and overall homology indices calculated from these data and chi-square testing of the results indicated that acid-soluble nuclear nonhistone proteins are more homologous than acid-insoluble nuclear proteins. Several factors which might have affected the results were discussed. |
キーワード | nuclear proteins protein homology polyacrylamide gel electrophoresis |
Amo Type | Article |
出版物タイトル | Acta Medica Okayama |
発行日 | 1985-04 |
巻 | 39巻 |
号 | 2号 |
出版者 | Okayama University Medical School |
開始ページ | 99 |
終了ページ | 104 |
ISSN | 0386-300X |
NCID | AA00508441 |
資料タイプ | 学術雑誌論文 |
言語 | 英語 |
論文のバージョン | publisher |
査読 | 有り |
PubMed ID | 4003115 |
Web of Science KeyUT | A1985AGK4600003 |
著者 | 筒井 公子| |
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発行日 | 1978-08 |
出版物タイトル | 岡山医学会雑誌 |
巻 | 90巻 |
号 | 7-8号 |
資料タイプ | 学術雑誌論文 |
著者 | 筒井 公子| |
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発行日 | 2009-12-01 |
出版物タイトル | 岡山医学会雑誌 |
巻 | 121巻 |
号 | 3号 |
資料タイプ | その他 |
著者 | 佐野 訓明| 宮地 まり| 筒井 公子| 筒井 研| |
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発行日 | 2009-12-01 |
出版物タイトル | 岡山医学会雑誌 |
巻 | 121巻 |
号 | 3号 |
資料タイプ | 学術雑誌論文 |
著者 | 筒井 公子| |
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発行日 | 2002-05-30 |
出版物タイトル | 岡山医学会雑誌 |
巻 | 114巻 |
号 | 1号 |
資料タイプ | その他 |
著者 | 筒井 公子| |
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発行日 | 2004-09-30 |
出版物タイトル | 岡山医学会雑誌 |
巻 | 116巻 |
号 | 2号 |
資料タイプ | 学術雑誌論文 |
著者 | 筒井 公子| |
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発行日 | 1978-03-31 |
出版物タイトル | |
資料タイプ | 学位論文 |