Dynamin is a protein essential to endocytosis. Dynamin 2, a dynamin isoform, is expressed most intensely in testicular tissue; however, precise localization has never been studied. Therefore, we investigated the expression of dynamin 2 in rat testicular tissue using immunohistochemical methods, and discuss here the physiological function of this protein. Testicular tissues were obtained from Wistar rats at 10, 21 and 63 days of age. Immunohistochemistrical examination and Western blot analysis were conducted using dynamin 2 specific antibody. Western blot analysis showed that expression in 21- and 63-day-old rats was more intense than that in 10-day-old rats. Dynamin 2 expression was observed using immunohistochemical method in the seminiferous tubules of all rats. In the 63-day-old rats, the expression was intense, especially in spermatids in the earlier maturation stages and in spermatocytes, and was observed in Sertoli cells. However, in spermatids, the expression gradually declined as spermatids matured to spermatozoa. In the 21-day-old rats, the expression was evident in spermatocytes and Sertoli cells, but that in the 10-day-old rats was weak. Intense expression of dynamin 2 during spermatogenesis suggests that this protein plays an important role in this process.
en-copyright= kn-copyright= en-aut-name=IguchiHiroki en-aut-sei=Iguchi en-aut-mei=Hiroki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WatanabeMasami en-aut-sei=Watanabe en-aut-mei=Masami kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KamitaniAkihiro en-aut-sei=Kamitani en-aut-mei=Akihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NagaiAtsushi en-aut-sei=Nagai en-aut-mei=Atsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HosoyaOsamu en-aut-sei=Hosoya en-aut-mei=Osamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TsutsuiKimiko en-aut-sei=Tsutsui en-aut-mei=Kimiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KumonHiromi en-aut-sei=Kumon en-aut-mei=Hiromi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University en-keyword=dynamin 2 kn-keyword=dynamin 2 en-keyword=endocytosis kn-keyword=endocytosis en-keyword=spermatogenesis kn-keyword=spermatogenesis END start-ver=1.4 cd-journal=joma no-vol=39 cd-vols= no-issue=2 article-no= start-page=99 end-page=104 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=198504 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Difference in the homology of two nuclear nonhistone protein fractions as compared by polyacrylamide gel electrophoresis. en-subtitle= kn-subtitle= en-abstract= kn-abstract=The extent of homology between two protein fractions was compared by simple electrophoretic analysis. Nuclear proteins of several rodent cells of different origins were fractionated into acid-soluble and acid-insoluble fractions. The two protein fractions were subjected to polyacrylamide gel electrophoresis in separate gel systems, and protein bands with identical mobilities were sought either in all possible combinational pairs of cell types or in all cell types. The paired and overall homology indices calculated from these data and chi-square testing of the results indicated that acid-soluble nuclear nonhistone proteins are more homologous than acid-insoluble nuclear proteins. Several factors which might have affected the results were discussed.
en-copyright= kn-copyright= en-aut-name=TsutsuiKen en-aut-sei=Tsutsui en-aut-mei=Ken kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsutsuiKimiko en-aut-sei=Tsutsui en-aut-mei=Kimiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AoyamaKoji en-aut-sei=Aoyama en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OdaTakuzo en-aut-sei=Oda en-aut-mei=Takuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=nuclear proteins kn-keyword=nuclear proteins en-keyword=protein homology kn-keyword=protein homology en-keyword=polyacrylamide gel electrophoresis kn-keyword=polyacrylamide gel electrophoresis END start-ver=1.4 cd-journal=joma no-vol=90 cd-vols= no-issue=7-8 article-no= start-page=999 end-page=1013 dt-received= dt-revised= dt-accepted= dt-pub-year=1978 dt-pub=197808 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Purification and properties of a high molecular weight acid soluble nuclear protein from mouse ascites sarcoma cells kn-title=マウス腹水肉腫細胞核からの高分子量酸可溶性蛋白質の精製とその性質 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A high molecular weight acid soluble nuclear protein (HAN-1) was isolated in a electrophoretically homogeneous state from mouse ascites sarcoma cells (SR-C3H cells) by the exclusion chromatography in Sephacryl S-200 and the DEAE-Sephadex chromatography of the nuclear 0.2M H(2)SO(4) extract. The content of HAN-1 in SR-C3H nuclei was about 4.3% per total histone, the highest among the cell types tested. The molecular weight of HAN-1 was estimated to be 125,000 by SDS-polyacrylamide gel electrophoresis. The amino acid composition of HAN-1 was rich in glutamic acid, alanine, lysine and aspartic acid, the acidic/basic amino acid ratio 1.8. The in vitro RNA synthesizing activity of SR-C3H cell RNA polymerases Ⅰ and Ⅱ on a naked DNA template was differentially affected by HAN-1; the reaction with polymerase I was inhibited and that with polymerase Ⅱ stimulated. en-copyright= kn-copyright= en-aut-name=TsutsuiKimiko en-aut-sei=Tsutsui en-aut-mei=Kimiko kn-aut-name=筒井公子 kn-aut-sei=筒井 kn-aut-mei=公子 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部癌源研究施設生化学部門 END start-ver=1.4 cd-journal=joma no-vol=121 cd-vols= no-issue=3 article-no= start-page=227 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2009 dt-pub=20091201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=To the memory of Professor Kahee Niimi kn-title=新見 嘉兵衛先生を偲んで en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TsutsuiKimiko M . en-aut-sei=Tsutsui en-aut-mei=Kimiko M . kn-aut-name=筒井公子 kn-aut-sei=筒井 kn-aut-mei=公子 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 神経ゲノム学（旧解剖学第三講座） END start-ver=1.4 cd-journal=joma no-vol=121 cd-vols= no-issue=3 article-no= start-page=143 end-page=147 dt-received= dt-revised= dt-accepted= dt-pub-year=2009 dt-pub=20091201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=DNA topoisomerase Ⅱβ activates a subset of neuronal genes kn-title=DNAトポイソメラーゼⅡβによる神経関連遺伝子の活性化機構 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=SanoKuniaki en-aut-sei=Sano en-aut-mei=Kuniaki kn-aut-name=佐野訓明 kn-aut-sei=佐野 kn-aut-mei=訓明 aut-affil-num=1 ORCID= en-aut-name=MiyajiMary en-aut-sei=Miyaji en-aut-mei=Mary kn-aut-name=宮地まり kn-aut-sei=宮地 kn-aut-mei=まり aut-affil-num=2 ORCID= en-aut-name=TsutsuiM. Kimiko en-aut-sei=Tsutsui en-aut-mei=M. Kimiko kn-aut-name=筒井公子 kn-aut-sei=筒井 kn-aut-mei=公子 aut-affil-num=3 ORCID= en-aut-name=TsutsuiKen en-aut-sei=Tsutsui en-aut-mei=Ken kn-aut-name=筒井研 kn-aut-sei=筒井 kn-aut-mei=研 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 神経ゲノム学 affil-num=2 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 神経ゲノム学 affil-num=3 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 神経ゲノム学 affil-num=4 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 遺伝情報動態学 en-keyword=DNAトポイソメラーゼⅡβ (DNA topoisomeraseⅡbeta) kn-keyword=DNAトポイソメラーゼⅡβ (DNA topoisomeraseⅡbeta) en-keyword=神経細胞分化 (neuronal differentiation) kn-keyword=神経細胞分化 (neuronal differentiation) en-keyword=遺伝子発現 (gene expression) kn-keyword=遺伝子発現 (gene expression) en-keyword=神経関連遺伝子 (neuronal gene) kn-keyword=神経関連遺伝子 (neuronal gene) en-keyword=クロマチン (chromatin) kn-keyword=クロマチン (chromatin) END start-ver=1.4 cd-journal=joma no-vol=114 cd-vols= no-issue=1 article-no= start-page=67 end-page=69 dt-received= dt-revised= dt-accepted= dt-pub-year=2002 dt-pub=20020530 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=徳永叡教授と脳の研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=筒井公子 kn-aut-sei=筒井 kn-aut-mei=公子 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=脳神経制御学講座神経機能構造学 END start-ver=1.4 cd-journal=joma no-vol=116 cd-vols= no-issue=2 article-no= start-page=103 end-page=107 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20040930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Ⅱ型 DNAトポイソメラーゼと神経細胞分化 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=筒井公子 kn-aut-sei=筒井 kn-aut-mei=公子 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯学総合研究科 神経機能構造学 en-keyword=Ⅱ型DNAトポイソメラーゼ kn-keyword=Ⅱ型DNAトポイソメラーゼ en-keyword=神経細胞分化 kn-keyword=神経細胞分化 en-keyword=遺伝子発現誘導 kn-keyword=遺伝子発現誘導 en-keyword=初代培養 kn-keyword=初代培養 en-keyword=シナプスタンパク質 kn-keyword=シナプスタンパク質 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1978 dt-pub=19780331 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=マウス腹水肉腫細胞核からの高分子量酸可溶性蛋白質の精製とその性質 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=筒井公子 kn-aut-sei=筒井 kn-aut-mei=公子 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END