ID | 18233 |
Eprint ID | 18233
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フルテキストURL | |
タイトル(別表記) | On the Properties of an Established Cell Strain, JTC-11, from Ehrlich Ascites Tumor in Tissue Culture Part 1 The Characteristics of the Standard Strain (K-Strain)
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著者 |
浜崎 充彦
岡山大学医学部微生物学教室
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抄録 | Ehrlich Ascites Tumor Cells (JTC-11) reported here have been cultured in vitro for more than 3 years by pipetting, using 80% YLE+20% Bovine serum (final concentration: yeast exract 0.08%, lactalbumin hydrolysate 0.4%) as the medium. As the results of tissue culture experiments concerning the characteristics of this strain, I obtained the following conclusion. 1) This (JTC-11) K-Strain is the easiest group of acutual animal malignant tissue culture strains in points of cell preservation, culture-method (pipetting), stability, medium-unchanging during one subculture term, inoculum size and adhesion on the glass surface. 2) The proliferation rate is the greatest (about 2000 times for 8 days) and even a small number of cells have the ability of proliferation. 3) After the intraperitoneal reinoculation into female mice with more than 1.6×10(4) this strain cells, all the mice died by the tumor. Accordingly tumor producing capacity has been strongly preserved after a lapse of 3 years. 4) The strain-character (Sato) of this K-strain cells has still remained after in vivo 100 days transplantion through Cb-mice (Experiments of retissue-culture). 5) This K-strain cells have a interesting form, namely the small sucker-form podia appear at the tip of the processes.
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発行日 | 1964-03-30
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出版物タイトル |
岡山医学会雑誌
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出版物タイトル(別表記) | Journal of Okayama Medical Association
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巻 | 76巻
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号 | 1-3号
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出版者 | 岡山医学会
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出版者(別表記) | Okayama Medical Association
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開始ページ | 1
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終了ページ | 11
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ISSN | 0030-1558
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NCID | AN00032489
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資料タイプ |
学術雑誌論文
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オフィシャル URL | https://www.jstage.jst.go.jp/article/joma1947/76/1-3/76_1-3_1/_article/-char/ja/
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関連URL | http://www.okayama-u.ac.jp/user/oma/
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言語 |
日本語
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著作権者 | 岡山医学会
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論文のバージョン | publisher
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査読 |
有り
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Eprints Journal Name | joma
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