鶏白血病腫瘍の酵素的性状 第2篇 Cell free exrtactについて

With the tumor tissue (node) developing in the liver of chicken with leukemia of visceral type and the tissue around the node as materials to be tested, and normal liver tissue as control, tissues were minced and cell free extracts were obtained by centrifugation procedures. Then the enzymic activities at the level of these cell free extracts were compared and the following results were obtained. 1. Succinoxidase is contained in the precipitate fraction centrifuged at 40,000 r.p.m. of the cell free extract while other enzymes are in the supernatant. 2. None of these cell free extracts show glycolysis and cancer cell extract does not reveal Crabtree effect either. 3. The prominent feature of cancer cells is that cells appear to be in an intact state but when they are destroyed, the difference between cancer cells and normal cells seems to be lost.