ID | 40758 |
フルテキストURL | |
タイトル(別表記) | Study of the reduction of mercuric ion by (L)-ascorbic acid, (DL)-α-tocopherol, superoxide anion and the supernatant of mouse liver homogenate - Experiment of the oxidation or reduction of mercury in vitro and in vivo - part 1
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著者 |
倉橋 康二郎
岡山大学医学部公衆衛生学教室
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抄録 | The reduction of (203)Hg(++) by (L)-ascorbic acid, (DL)-α-tocopherol, superoxide anion and the supernatant of mouse liver homogenate after the ultracentrifugation were performed. The results obtained were as follows. 1. The relationships between the reductive rate of mercuric ion and the concentration of the (L)-AsA or (DL)-α-tocopherol were shown as a sigmoidal curve; that of the superoxide anion was a linear. 2. The activity of reduction of Hg(++) was in decreasing order with (L)-AsA, (DL)-α-tocopherol, and superoxide anion, respectively. 3. The liver homogenate was used with 8.5% sucrose and 11% polyvinylpirrolidone (PVP) solution with preparation of the homogenate to prevent release of catalase from the peroxisomes. Another preparation used 44mM phosphate buffer (pH 6.8) solution and the liver catalase was released from peroxisomes. The reductive rate of mercuric ion with the former preparation was 6.4 times higher than with the latter. It indicates that the reoxidation of metallic mercury by the catalase reduced the reductive rate of mercuric ion. 4. A reductive experiment of mercuric ion by liver supernatant containing sucrose and PVP at 0°C and 37°C was performed. The reduction rate by this sample at 0°C is 1/5.48 of that at 37° C. This suggests that the reduction of mercuric ion in liver homogenate is due to an enzymatic reaction.
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キーワード | reduction of mercuric ion
(L)-ascorbic acid
(DL)-α-tocopherol
superoxide anion
liver homogenate
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発行日 | 1989-06
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出版物タイトル |
岡山医学会雑誌
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出版物タイトル(別表記) | Journal of Okayama Medical Association
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巻 | 101巻
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号 | 5-6号
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出版者 | 岡山医学会
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出版者(別表記) | Okayama Medical Association
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開始ページ | 603
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終了ページ | 612
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ISSN | 0030-1558
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NCID | AN00032489
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資料タイプ |
学術雑誌論文
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オフィシャル URL | https://www.jstage.jst.go.jp/article/joma1947/101/5-6/101_5-6_603/_article/-char/ja/
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関連URL | http://www.okayama-u.ac.jp/user/oma/
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言語 |
日本語
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著作権者 | 岡山医学会
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論文のバージョン | publisher
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査読 |
有り
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Eprints Journal Name | joma
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