ID | 11828 |
JaLCDOI | |
Sort Key | 6
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タイトル(別表記) | Measurement of Fe(2+)-induced Lipid Peroxidation of Mitochondria used Thiobarbituric Acid Method under the Acetic Acid Conditions
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フルテキストURL | |
著者 |
山本 剛禧
岡山大学医療技術短期大学部診療放射線技術学科
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抄録 | Mitochondrial lipid peroxidations were usually detected by the thiobarbituric acid (TBA) color reaction under the acidic conditions with hydrochloric and trichloroacetic acids. The time response curve of Fe(2+)-induced lipid peroxidation, accompanied the lag time of induction period, of mouse liver mitochondria was measured with detection of the produced lipid peroxides by the TBA method under the acidic conditions with acetic acid. Fe(2+)-induced lipid peroxidation was terminated by the addition of ethylenediaminetetraacetate (EDTA). The lag time of the peroxidation detected by TBA method in the acetic acid conditions was obtained as well as that detected in the hydrochloric and trichloroacetic acids conditions. The fact indicates that Fe(2+)-induced lipid peroxidation of mitochondria and its lipid peroxides are able to be measured by the TBA color reaction in the acetic acid conditions with EDTA.
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キーワード | Mitochondrial Lipid Peroxidation
Thiobarbituric Acid
Ferrous Ion
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出版物タイトル |
岡山大学医療技術短期大学部紀要
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発行日 | 1993-01-31
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巻 | 3巻
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出版者 | 岡山大学医療技術短期大学部
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出版者(別表記) | School of Health Sciences Okayama University
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開始ページ | 43
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終了ページ | 47
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ISSN | 0917-4494
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NCID | AN10355371
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資料タイプ |
紀要論文
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OAI-PMH Set |
岡山大学
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言語 |
日本語
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論文のバージョン | publisher
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NAID | |
Eprints Journal Name | fhs
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