Acta Medica Okayama 22巻 3号

A report is made on a case of liposarcoma of stomach in a 42 year old man. This is the first case of liposarcoma of stomach reported in Japan. The patient has remained asymptomatic for five years after operation.

1. For the purpose to clarify the mechanism of the revolutional changes in energy metabolism during the reticulocyte maturation the metabolisms of glucose and of the pentose moieties of acid soluble nucleotides have been observed on rabbit reticulocytes incubated in vitro under various conditions. 2. The maturation of reticulocyte proceeds by using the energy produced by aerobic glycolysis and is arrested in the glucose deficient medium, but the pentose moieties of purine nucleotide and nucleoside added exogenously serve as the energy source for reticulocyte maturation even in the absence of glucose. 3. The test on the utility efficiency of glucose and inosine as the energy source for reticulocyte maturation revealed that glucose is used more effectively than the pentose moiety of inosine under aerobic condition, which is advantageous for reticulocyte maturation, and vice versa under anaerobic condition, which is comparable to the metabolism of mature red cell. 4. From these results it has been suggested that the maturation of reticulocyte is the process of degradation of RNA and acid soluble nucleotides supported by the aerobic glycolysis, where the degradation products of RNA and acid soluble purine nucleotides provide the purine derivatives as the material for ATP synthesis (36) and the pentose moieties as energy source. 5. A possible mechanism for the superior utility of glucose to nucleoside pentose during reticulocyte maturation and vice versa in mature red cell has been discussed.

Replacement of abdominal vena cava with a fresh autogenous substitute, the segment of small intestinal submucosa, was attempted in 15 animals. Five segments were prepared from the intestine smeared with iodine tincture, and reinforced with a steel coil externally in the entire length and a steel or polyethylene ring at the anastomosis. Thoracoabdominal long implantation was done in three animals, of which one with the intestinal segment devoid of mucosa, and the other two with the submucosa. Replacement of abdominal vena cava with the submucosa taken out of the intestinal segment preserved for nine days in 1% mercurochrome solution, or 0.1 % acrinolum solution was done in one animal each. In these two a coil and two rings were also applied. Replacement of abdominal aorta with the double layer tube of a reconstructed submucosa and another very porous Tetoron gauze was done in two animals, each coupled with the abdominal vena cava replacement at the same time. Of these experiments, aorta replacements were nearly patent in both. The abdominal vena cava replacements made of the submucosa treated with iodine tincture were patent in three. The one that was preserved in acrinolum showed moderate constriction. Most of the others were also observed for a long period of time but these all occluded in spite of the initial patency which was revealed at three to seven days in cavograms, and the time of the occlusion was not determined. The internal surface of the segment of submucosa, being implanted, is covered in the first stage with the deposition of fibrin, which is subsequently organized into a fibrous layer, in the same manner as that of the synthetic graft. Another disadvantage of this substitute is its readily collapsible tendency. Infection is preventable in the experiment. The substitute seems to be useful for the replacement of aorta and for the short segment of vena cave.

For the column chromatographic isolation of individual phospholipids from the total phospholipid mixture, silicic acid, DEAE cellulose, alumina and others, have been used as adsorbent. However, it must be emphasized that silicic acid (1, 2, 3, 4) is the most useful adsorbent for the separation of the total phospholipid mixture from each other in reasonable purity. VAN DEENEN reported that pure phosphatidyl glycerol was obtained from the lipid fraction of spinach leaves after repeated chromatography on silicic acid column (5). The phospholipid extracted from Escherichia coli B consists of abundant phosphatidyl ethanolamine (70-80 %), cardiolipin, phosphatidyl glycerol and other minor components as described in the previous paper (6). The high percentage content of phosphatidyl ethanolamine renders it difficult to separate the phospholipids by the column chromatography. Therefore, repeated chromatographies on the silicic acid column treated with sodium bicarbonate (7) and normal silicic acid column were employed for the isolation of the major components from the total phospholipid of E. coli B. Stepwise elution (4) was carried out with chloroform containing increasing proportions of methanol, and the eluent was divided into several fractions according to experience with thin-layer chromatography.

The rats which received the repeated intra peritoneal or intravenous　injections of methyl palmitate showed a marked depressed phagocytic activity　of the RES as shown by the clearance test with radioactive iron as　well as by histological observations and a significantly suppressed antibody　formation against the challenge by BSA.　Differing from the cases of the blockade of the RES made by PVP or　radiogold, the injection of methyl palmitate did not result in any injurious　effect on the lymph follicles of lymph nodes and spleen and the plasma　cells proliferation as revealed by the histological observation. Histochemical observations of iron phagocytosis of the RES done by　Perls stain revealed that methyl palmitate　suppressed the phagocytic activity　of the Kupffer cells of the liver dramatically and also suppressed the　phagocytic activity of the sinus-lining cells in spleen to a lesser degree.　The result indicates that the injection of methyl palmitate attacks the phagocytic　function of the RES selectively and induces the reduced immune　response of the organism without giving any damages to the proliferation of immunologically com petent cells.　The fact suggests that the RES lowered in their phagocytic activity　fails to produce the informational substance for immune　response, showing　a lower level in the antibody formation even in the presence of antigen　and proliferating immunologically competent cells.