The supernatant of the regional lymph node of mice transplanted with Ehrlich ascites tumor were fractionated on DEAE Sephadex A-50 column chromatography with linear gradient elution. The eluted peaks were examined electrophretically. They were γ, β. α and albumin. β and α fractions were effective for suppression of Ehrlich ascites cell growth. Minimum effective β fraction was 300μg/ml. The sedimentation coefficient of β fraction was 6.5 S 20 w, and that of α fraction was 7.4 S 20 w.