Activity and stability of catalase in the organs of acatalasemic mice-Comparison of activities at different incubating temperatures by the perborate method

Catalase activities in the organs of normal, acatalasemic and heterozygous-hypocatalasemic mice were determined after the tissues were incubated with perborateat different temperatures. Catalase activity in the normal mouse hemolysate and liver homogenate measured after being incubated with perborate at 20℃ for 5 min. was slightly lower than that measured at 37℃. In contrast, residual catalase activity in acatalasemic mouse hemolysate and liver homogenate measured at 20℃ for 5 min. was markedly higher than that measured at 37℃, suggesting that residual catalase in the acatalasemic mouse blood and liver is inactivated during incubation with perborate at 37℃ for 5 min. Catalase activity in the blood and solid tissues of acatalasemic mice measured at 20℃ was 1.29 to 3.30 times as high as that measured at 37℃. The ratio of catalase activity in the blood and solid tissues of acatalasemic mice to that in normal mouse tissues incubated with perborate at 20℃ for 5 min. was calculated to be 4.3% in blood, 65.7% in liver, 15.6% in kidneys, 69.9% in lungs, 33.7% in stomach, 30.0% in brain and 31.8% in heart. Catalase activity in the blood and solid tissues in heterozygous hypocatalasemic mice measured at 20℃ was 1.09 to 1.70 times as high as that measured at 37℃. This ratio of catalase activity in the heterozygous hypocatalasemic mouse tissue incubated at 20℃ to the activity of tissue incubated at 37℃ is between the ratio of normal mice and that of acatalasemic mice. The ratio of catalase activity in the blood and solid tissues of heterozygous hypocatalasemic mice to the activity in the corresponding organs of normal mice measured at 20℃ for 5 min. was calculated to be 47.3% in blood, 95.0% in liver, 36.6% in kidneys, and 44.9% in stomach.