Journal of Okayama Medical Association
Published by Okayama Medical Association

<Availability>
Full-text articles are available 3 years after publication.

アカタラセミアマウス及びヒポカタラセミアマウスの臓器中のカタラーゼの活性度及び安定度(過ホウ素酸法による各種の温度ふ置後の測定による活性度の比較)

鳥取 美男 岡山大学公衆衛生学教室
Thumnail 99_1623.pdf 487 KB
抄録
Catalase activities in the organs of normal, acatalasemic and heterozygous-hypocatalasemic mice were determined after the tissues were incubated with perborateat different temperatures. Catalase activity in the normal mouse hemolysate and liver homogenate measured after being incubated with perborate at 20℃ for 5 min. was slightly lower than that measured at 37℃. In contrast, residual catalase activity in acatalasemic mouse hemolysate and liver homogenate measured at 20℃ for 5 min. was markedly higher than that measured at 37℃, suggesting that residual catalase in the acatalasemic mouse blood and liver is inactivated during incubation with perborate at 37℃ for 5 min. Catalase activity in the blood and solid tissues of acatalasemic mice measured at 20℃ was 1.29 to 3.30 times as high as that measured at 37℃. The ratio of catalase activity in the blood and solid tissues of acatalasemic mice to that in normal mouse tissues incubated with perborate at 20℃ for 5 min. was calculated to be 4.3% in blood, 65.7% in liver, 15.6% in kidneys, 69.9% in lungs, 33.7% in stomach, 30.0% in brain and 31.8% in heart. Catalase activity in the blood and solid tissues in heterozygous hypocatalasemic mice measured at 20℃ was 1.09 to 1.70 times as high as that measured at 37℃. This ratio of catalase activity in the heterozygous hypocatalasemic mouse tissue incubated at 20℃ to the activity of tissue incubated at 37℃ is between the ratio of normal mice and that of acatalasemic mice. The ratio of catalase activity in the blood and solid tissues of heterozygous hypocatalasemic mice to the activity in the corresponding organs of normal mice measured at 20℃ for 5 min. was calculated to be 47.3% in blood, 95.0% in liver, 36.6% in kidneys, and 44.9% in stomach.
キーワード
アカタラセミアマウス
ヒポカタラセミアマウス
カタラーゼ活性度
カタラーゼ安定度
カタラーゼ測定法
ISSN
0030-1558
NCID
AN00032489