Studies on Influence of Edta to Bacteria Part II Enzymatic Properties of Bacteria Grown on EDTA Added Media

Using Sal. 57 S, the auther carried out the investigation on the enzymatic properties of EDTA resistant bacteria grown on EDTA added nutrient agar media in comparison to normal Sal. 57S. The following results were obtained 1) On oxidatinn of glucose, the normal bacteria oxidized it mostly through the EMBDEN-MEYERHOF pathway. But in the EDTA resistant bacteria the oxidaition pathway was supposedly via WARBURG-DICKENS shunt, While there were evidence of passing through the EMBDEN-MEYERHOR pathway to some extent. 2) Further oxidation beyond pyrvate stage was strongly inhibited in EDTA resiseant bacteria. 3) The enzymatic change mentioned above was easily restored to the normal by the shrot time incubation of the organism. 4) It was postulated no change occured in the hydrogen transfer system.