Journal of Okayama Medical Association
Published by Okayama Medical Association

Full-text articles are available 3 years after publication.

EDTAの細菌に対する影響に関する研究 第2編 EDTA加培養基に発育せる細菌の酵素的性状に就いて

茎田 芳明 岡山大学医学部微生物学教室
71_6563.pdf 421 KB
Using Sal. 57 S, the auther carried out the investigation on the enzymatic properties of EDTA resistant bacteria grown on EDTA added nutrient agar media in comparison to normal Sal. 57S. The following results were obtained 1) On oxidatinn of glucose, the normal bacteria oxidized it mostly through the EMBDEN-MEYERHOF pathway. But in the EDTA resistant bacteria the oxidaition pathway was supposedly via WARBURG-DICKENS shunt, While there were evidence of passing through the EMBDEN-MEYERHOR pathway to some extent. 2) Further oxidation beyond pyrvate stage was strongly inhibited in EDTA resiseant bacteria. 3) The enzymatic change mentioned above was easily restored to the normal by the shrot time incubation of the organism. 4) It was postulated no change occured in the hydrogen transfer system.