1) In the dog, as to the bactericidal action, the blood of the femoral, pulmonary and hepatic veins are stronger than those of the femoral artery, pulmonary artery and the portal vein respectively. Among them the serum bactericidal action of the blood of the hepatic vein is the strongest. 2) In the human, the serum bactericidal actions of the blood of the cubital vein and pulmonary vein is stronger than that of the brachial artery and pulmonary artery. 3) The amount of serum γ-globulin is greater in the blood of the cubital vein and pulmonary vein than in that of the brachial artery and pulmonary artery respectively, prsssnting a correlation with the difference of serum bactericidal actions, thus suggesting their important roles in the bactericidal action. 4) The blood sugar level is a little higher in the blood of the brachial artery, femoral artery and pulmonary artery than in that of the cubital vein, pulmonary vein and femoral vein respectively; however, the blood sugar content of the hepatic vein and portal vein does not give a constant value. Consequently the blood sugar level in various vessels does not seem to have any significant relation to the difference of the serum bactericidal effect.